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SDS-Page (Gel: 12%SDS-PAGE, Lysate: 40 μg, Lane 1-4: Mouse liver tissue, HepG2, 293T and 231 cell lysates, Primary antibody:47675(PEMT Antibody) at dilution 1/800, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 1 second)

Rabbit anti-Human, Mouse PEMT Polyclonal Antibody | anti-PEMT antibody

PEMT Antibody

Gene Names
PEMT; PLMT; PNMT; PEAMT; PEMPT; PEMT2
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
Antigen affinity purification
Synonyms
PEMT; Polyclonal Antibody; PEMT Antibody; PLMT; PNMT; PEAMT; PEMPT; PEMT2; anti-PEMT antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
The antibody detects endogenous levels of total PEMT protein.
Purity/Purification
Antigen affinity purification
Form/Format
pH 7.4 PBS, 0.05% NaN3, 40% Glycerol
Concentration
1.6 mg/mL (varies by lot)
Sequence Length
214
Applicable Applications for anti-PEMT antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:1000-5000
IHC: 1:50-300
Immunogen
Synthetic peptide of human PEMT
Immunogen Type
Peptide
Preparation and Storage
Store at -20 degree C.

SDS-Page

(Gel: 12%SDS-PAGE, Lysate: 40 μg, Lane 1-4: Mouse liver tissue, HepG2, 293T and 231 cell lysates, Primary antibody:47675(PEMT Antibody) at dilution 1/800, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 1 second)

SDS-Page (Gel: 12%SDS-PAGE, Lysate: 40 μg, Lane 1-4: Mouse liver tissue, HepG2, 293T and 231 cell lysates, Primary antibody:47675(PEMT Antibody) at dilution 1/800, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 1 second)

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using 47675(PEMT Antibody) at dilution 1/55.(Original magnification: 200))

Immunohistochemistry (IHC) (The image is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using 47675(PEMT Antibody) at dilution 1/55.(Original magnification: 200))
Related Product Information for anti-PEMT antibody
Phosphatidylcholine (PC) is the most abundant mammalian phospholipid. This gene encodes an enzyme which converts phosphatidylethanolamine to phosphatidylcholine by sequential methylation in the liver. Another distinct synthetic pathway in nucleated cells converts intracellular choline to phosphatidylcholine by a three-step process. The protein isoforms encoded by this gene localize to the endoplasmic reticulum and mitochondria-associated membranes. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms.
Product Categories/Family for anti-PEMT antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
22 kDa
NCBI Official Full Name
phosphatidylethanolamine N-methyltransferase isoform 4
NCBI Official Synonym Full Names
phosphatidylethanolamine N-methyltransferase
NCBI Official Symbol
PEMT
NCBI Official Synonym Symbols
PLMT; PNMT; PEAMT; PEMPT; PEMT2
NCBI Protein Information
phosphatidylethanolamine N-methyltransferase
UniProt Protein Name
Phosphatidylethanolamine N-methyltransferase
UniProt Gene Name
PEMT
UniProt Synonym Gene Names
PEMPT; PNMT; PEAMT

NCBI Description

Phosphatidylcholine (PC) is the most abundant mammalian phospholipid. This gene encodes an enzyme which converts phosphatidylethanolamine to phosphatidylcholine by sequential methylation in the liver. Another distinct synthetic pathway in nucleated cells converts intracellular choline to phosphatidylcholine by a three-step process. The protein isoforms encoded by this gene localize to the endoplasmic reticulum and mitochondria-associated membranes. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2012]

Uniprot Description

Catalyzes the three sequential steps of the methylation pathway of phosphatidylcholine biosynthesis, the SAM-dependent methylation of phosphatidylethanolamine (PE) to phosphatidylmonomethylethanolamine (PMME), PMME to phosphatidyldimethylethanolamine (PDME), and PDME to phosphatidylcholine (PC).

Research Articles on PEMT

Similar Products

Product Notes

The PEMT pemt (Catalog #AAA9430657) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PEMT Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PEMT can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:1000-5000 IHC: 1:50-300. Researchers should empirically determine the suitability of the PEMT pemt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PEMT, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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