Human golgi membrane protein 1 ELISA Kit | GOLM1 elisa kit

Human golgi protein 73, GP-73 ELISA Kit

Cat. Num. AAA704553

• Gene Names: GOLM1; GP73; HEL46; GOLPH2; C9orf155; PSEC0257; bA379P1.3
• Reactivity: Human
• Synonyms: golgi membrane protein 1; Human golgi protein 73; GP-73 ELISA Kit; RP11-379P1.3; C9orf155; FLJ22634; FLJ23608; GOLPH2; GP73; PSEC0257; bA379P1.3; golgi membrane protein GP73; golgi phosphoprotein 2; golgi protein; 73-kD; GOLM1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human GP-73. No significant cross-reactivity or interference between human GP-73 and analogues was observed.
• Sequence Length: 401

• Samples: Serum, plasma, cell culture supernates and tissue homogenates
• Assay Type: Sandwich
• Detection Range: 0.156 IU/ml -10 IU/ml.
• Sensitivity: The minimum detectable dose of human GP-73 is typically less than 0.039 IU/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GOLM1 elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GP-73 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GP-73 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GP-73 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GP-73 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 29550838
• NCBI GeneID: 51280
• NCBI Accession #: NP_057632.2
• NCBI GenBank Nucleotide #: NM_016548.3
• UniProt Accession #: Q8NBJ4; Q6IAF4; Q9NRB9
• Molecular Weight: 45,333 Da
• NCBI Official Full Name: Golgi membrane protein 1
• NCBI Official Synonym Full Names: golgi membrane protein 1
• NCBI Official Symbol: GOLM1
• NCBI Official Synonym Symbols: GP73; HEL46; GOLPH2; C9orf155; PSEC0257; bA379P1.3
• NCBI Protein Information: Golgi membrane protein 1; golgi protein, 73-kD; golgi phosphoprotein 2; golgi membrane protein GP73; epididymis luminal protein 46
• UniProt Protein Name: Golgi membrane protein 1
• Protein Family: Golgi membrane protein
• UniProt Gene Name: GOLM1
• UniProt Synonym Gene Names: C9orf155; GOLPH2; PSEC0242; UNQ686/PRO1326
• UniProt Entry Name: GOLM1_HUMAN

NCBI Description

The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a type II Golgi transmembrane protein. It processes proteins synthesized in the rough endoplasmic reticulum and assists in the transport of protein cargo through the Golgi apparatus. The expression of this gene has been observed to be upregulated in response to viral infection. Alternatively spliced transcript variants encoding the same protein have been described for this gene. [provided by RefSeq, Sep 2009]

Uniprot Description

Function: Unknown. Cellular response protein to viral infection.