Rabbit HDAC2 Polyclonal Antibody | anti-HDAC2 antibody

HDAC2 Polyclonal Antibody

Cat. Num. AAA125275

• Gene Names: HDAC2; HD2; RPD3; YAF1
• Reactivity: Human, mouse, rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity purification
• Synonyms: HDAC2; Polyclonal Antibody; HDAC2 Polyclonal Antibody; HDAC2 ; RPD3; YAF1 ;; anti-HDAC2 antibody

• Host: Rabbit
• Reactivity: Human, mouse, rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purification
• Sequence Length: 488

• Applicable Applications for anti-HDAC2 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Western Blot (WB): 1:500 - 1:2000; Immunohistochemistry (IHC): 1:50 - 1:200; Immunofluorescence (IF): 1:20 - 1:50
• Immunogen: A fusion protein of human HDAC2
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Store at -20 degree C or -80 degree C. Avoid freeze / thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of H460 cells, using HDAC2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)

Related Product Information for anti-HDAC2 antibody

Acetylation of the histone tail causes chromatin to adopt an "open" conformation, allowing increased accessibility of transcription factors to DNA. The identification of histone acetyltransferases (HATs) and their large multiprotein complexes has yielded important insights into how these enzymes regulate transcription (1,2). HAT complexes interact with sequencespecific activator proteins to target specific genes. In addition to histones, HATs can acetylate nonhistone proteins, suggesting multiple roles for these enzymes (3). In contrast, histone deacetylation promotes a "closed" chromatin conformation and typically leads to repression of gene activity (4). Mammalian histone deacetylases can be divided into three classes on the basis of their similarity to various yeast deacetylases (5). Class I proteins (HDACs 1, 2, 3, and 8) are related to the yeast Rpd3like proteins, those in class II (HDACs 4, 5, 6, 7, 9, and 10) are related to yeast Hda1like proteins, and class III proteins are related to the yeast protein Sir2. Inhibitors of HDAC activity are now being explored as potential therapeutic cancer agents (6,7). HDAC1 and HDAC2 are highly homologous and are involved in histone deacetylation, chromatin remodeling and transcriptional repression (810). Both proteins are found together in numerous complexes including the nucleosome remodeling and deacetylation complex (NuRD), MeCP1, and the mSin3A corepressor complex.

References

1. Marmorstein, R. (2001) Cell Mol Life Sci 58, 693-703. 2. Gregory, P.D. et al. (2001) Exp Cell Res 265, 195-202. 3. Liu, Y. et al. (2000) Mol Cell Biol 20, 5540-53. 4. Cress, W.D. and Seto, E. (2000) J Cell Physiol 184, 1-16. 5. Gray, S.G. and Ekström, T.J. (2001) Exp Cell Res 262, 75-83. 6. Thiagalingam, S. et al. (2003) Ann. N.Y. Acad. Sci. 983, 84-100. 7. Vigushin, D.M. and Coombes, R.C. (2004) Curr. Cancer Drug Targets 4, 205-218. 8. Zhang, Y. et al. (1999) Genes Dev. 13, 1924-1935. 9. Ng, H.H. et al. (1999) Nat. Genet. 23, 58-61. 10. Zhang, Y. et al. (1997) Cell 89, 357-364.

NCBI and Uniprot Product Information

• NCBI GI #: 293336691
• NCBI GeneID: 3066
• NCBI Accession #: NP_001518.3
• NCBI GenBank Nucleotide #: NM_001527.3
• UniProt Accession #: Q92769; Q5SRI8; Q5SZ86; Q8NEH4; B4DL58; E1P561
• Molecular Weight: 55kDa
• NCBI Official Full Name: histone deacetylase 2
• NCBI Official Synonym Full Names: histone deacetylase 2
• NCBI Official Symbol: HDAC2
• NCBI Official Synonym Symbols: HD2; RPD3; YAF1
• NCBI Protein Information: histone deacetylase 2; YY1-associated factor 1; transcriptional regulator homolog RPD3
• UniProt Protein Name: Histone deacetylase 2
• UniProt Gene Name: HDAC2
• UniProt Synonym Gene Names: HD2
• UniProt Entry Name: HDAC2_HUMAN