Mouse tachykinin receptor 1 ELISA Kit | TACR1 elisa kit

Mouse substance P receptor, SP-R ELISA Kit

Cat. Num. AAA703311

• Gene Names: Tacr1; Spr; Nk1r; Tac1r
• Reactivity: Mouse
• Synonyms: tachykinin receptor 1; Mouse substance P receptor; SP-R ELISA Kit; NK1R; NKIR; SPR; TAC1R; NK-1 receptor; neurokinin 1 receptor; tachykinin 1 receptor (substance P receptor; neurokinin 1 receptor) ; tachykinin receptor 1 (substance P receptor; neurokinin-1 receptor) ; TACR1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse SP-R. No significant cross-reactivity or interference between mouse SP-R and analogues was observed.
• Sequence Length: 407

• Samples: Serum, plasma, Cell lysates, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 7.8 pg/ml-500 pg/ml.
• Sensitivity: The minimum detectable dose of mouse SP-R is typically less than 1.95 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TACR1 elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SP-R has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SP-R present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SP-R is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SP-R bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 31543840
• NCBI GeneID: 21336
• NCBI Accession #: NP_033339.2
• NCBI GenBank Nucleotide #: NM_009313.5
• UniProt Accession #: P30548; Q8BYR7
• Molecular Weight: 46,322 Da
• NCBI Official Full Name: substance-P receptor
• NCBI Official Synonym Full Names: tachykinin receptor 1
• NCBI Official Symbol: Tacr1
• NCBI Official Synonym Symbols: Spr; Nk1r; Tac1r
• NCBI Protein Information: substance-P receptor; Nk1 receptor; substance P receptor; neurokinin receptor 1
• UniProt Protein Name: Substance-P receptor
• Protein Family: Substance-P receptor
• UniProt Gene Name: Tacr1
• UniProt Synonym Gene Names: Tac1r; SPR; NK-1R
• UniProt Entry Name: NK1R_MOUSE

NCBI Description

This gene encodes the receptor for the tachykinin, substance P, also referred to as neurokinin 1. This gene belongs to a gene family of tachykinin receptors which are characterized by interactions with G proteins and contain seven hydrophobic transmembrane regions. This receptor has been associated with nitric oxide formation, and it has been localized to cholinergic and nitrergic neurons as well as on smooth muscle cells. [provided by RefSeq, Mar 2010]

Uniprot Description

Function: This is a receptor for the tachykinin neuropeptide substance P. It is probably associated with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of affinity of this receptor to tachykinins is: substance P > substance K > neuromedin K.