Human glutathione peroxidase 4 (phospholipid hydroperoxidase) ELISA Kit | GPX4 elisa kit

Human Phospholipid hydroperoxide glutathione peroxidase, mitochondrial, GPX4 ELISA Kit

Cat. Num. AAA908073

• Gene Names: GPX4; MCSP; GPx-4; PHGPx; snGPx; GSHPx-4; snPHGPx
• Reactivity: Human
• Synonyms: glutathione peroxidase 4 (phospholipid hydroperoxidase); Human Phospholipid hydroperoxide glutathione peroxidase; mitochondrial; GPX4 ELISA Kit; mitochondrial (GPX4) ELISA kit; MCSP; PHGPx; snGPx; snPHGPx; glutathione peroxidase 4; phospholipid hydroperoxidase; phospholipid hydroperoxide glutathione peroxidase; sperm nucleus glutathione peroxidase; GPX4 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human GPX4. No significant cross-reactivity or interference between human GPX4 and analogues was observed.
• Sequence Length: 227

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 7.8 uU/ml-500 uU/ml.
• Sensitivity: The minimum detectable dose of human GPX4 is typically less than 1.95 uU/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GPX4 elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GPX4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GPX4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GPX4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GPX4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 90903238
• NCBI GeneID: 2879
• NCBI Accession #: NP_001034936.1
• NCBI GenBank Nucleotide #: NM_001039847.2
• UniProt Accession #: P36969; O43381; Q6PJ59; Q9UPK2
• Molecular Weight: 22,175 Da
• NCBI Official Full Name: phospholipid hydroperoxide glutathione peroxidase, mitochondrial isoform B
• NCBI Official Synonym Full Names: glutathione peroxidase 4
• NCBI Official Symbol: GPX4
• NCBI Official Synonym Symbols: MCSP; GPx-4; PHGPx; snGPx; GSHPx-4; snPHGPx
• NCBI Protein Information: phospholipid hydroperoxide glutathione peroxidase, mitochondrial; phospholipid hydroperoxidase; sperm nucleus glutathione peroxidase
• UniProt Protein Name: Phospholipid hydroperoxide glutathione peroxidase, mitochondrial
• Protein Family: Probable glutathione peroxidase
• UniProt Gene Name: GPX4
• UniProt Synonym Gene Names: PHGPx; GPx-4; GSHPx-4
• UniProt Entry Name: GPX4_HUMAN

NCBI Description

This gene encodes a member of the glutathione peroxidase protein family. Glutathione peroxidase catalyzes the reduction of hydrogen peroxide, organic hydroperoxide, and lipid peroxides by reduced glutathione and functions in the protection of cells against oxidative damage. Human plasma glutathione peroxidase has been shown to be a selenium-containing enzyme and the UGA codon is translated into a selenocysteine. The encoded protein has been identified as a moonlighting protein based on its ability to serve dual functions as a peroxidase as well as a structural protein in mature spermatozoa. Through alternative splicing and transcription initiation, rat produces proteins that localize to the nucleus, mitochondrion, and cytoplasm. In humans, alternative transcription initiation and the cleavage sites of the mitochondrial and nuclear transit peptides need to be experimentally verified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Uniprot Description

GPX4: Protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility. Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage. Belongs to the glutathione peroxidase family. 2 isoforms of the human protein are produced by alternative initiation.

Protein type: Nuclear envelope; Oxidoreductase; Mitochondrial; Lipid Metabolism - arachidonic acid; Other Amino Acids Metabolism - glutathione; EC 1.11.1.12

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: mitochondrion; nucleus; cytosol

Molecular Function: selenium binding; glutathione binding; phospholipid-hydroperoxide glutathione peroxidase activity; glutathione peroxidase activity

Biological Process: glutathione metabolic process; lipoxygenase pathway; hydrogen peroxide catabolic process; phospholipid metabolic process; multicellular organismal development; regulation of inflammatory response; arachidonic acid metabolic process; response to oxidative stress; response to estradiol stimulus; aging