Mouse insulin-like growth factor binding protein 3 ELISA Kit | IGFBP3 elisa kit

Mouse Insulin-like growth factor binding protein 3, IGFBP-3 ELISA Kit

Cat. Num. AAA702824

• Gene Names: Igfbp3; IGFBP-3; IGgfbp3; AI649005
• Reactivity: Mouse
• Synonyms: insulin-like growth factor binding protein 3; Mouse Insulin-like growth factor binding protein 3; IGFBP-3 ELISA Kit; IGFBP-3 ELISA KIT; tcag7.703; BP-53; IBP3; IGF-binding protein 3; acid stable subunit of the 140 K IGF complex; binding protein 29; binding protein 53; growth hormone-dependent binding protein; IGFBP-3; IGFBP3 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse IGFBP-3. No significant cross-reactivity or interference between mouse IGFBP-3 and analogues was observed.
• Sequence Length: 292

• Samples: Serum, plasma, tissue homogenates.
• Assay Type: Sandwich
• Detection Range: 0.391 ng/ml -25 ng/ml.
• Sensitivity: 0.196 ng/ml.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Related Product Information for IGFBP3 elisa kit

Principle of the Assay||This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IGFBP-3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IGFBP-3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IGFBP-3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IGFBP-3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 111378400
• NCBI GeneID: 16009
• NCBI Accession #: NP_032369.2
• NCBI GenBank Nucleotide #: NM_008343.2
• UniProt Accession #: P47878; Q6PE62
• Molecular Weight: 31,687 Da
• NCBI Official Full Name: insulin-like growth factor-binding protein 3
• NCBI Official Synonym Full Names: insulin-like growth factor binding protein 3
• NCBI Official Symbol: Igfbp3
• NCBI Official Synonym Symbols: IGFBP-3; IGgfbp3; AI649005
• NCBI Protein Information: insulin-like growth factor-binding protein 3; IBP-3; IGF-binding protein 3
• UniProt Protein Name: Insulin-like growth factor-binding protein 3
• Protein Family: Insulin-like growth factor-binding protein
• UniProt Gene Name: Igfbp3
• UniProt Synonym Gene Names: Igfbp-3; IBP-3; IGF-binding protein 3; IGFBP-3
• UniProt Entry Name: IBP3_MOUSE

Uniprot Description

Function: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R.