Typical Testing Data/Standard Curve (for reference only)
Human insulin-like growth factor binding protein 2, 36kDa ELISA Kit | IGFBP2 elisa kit
Human insulin-like growth factors binding protein 2, IGFBP-2 ELISA Kit
Gene Names
IGFBP2; IBP2; IGF-BP53
Reactivity
Human
Synonyms
insulin-like growth factor binding protein 2; 36kDa; Human insulin-like growth factors binding protein 2; IGFBP-2 ELISA Kit; IBP2; IGF-BP53; ; IGFBP2 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human IGFBP2. No significant cross-reactivity or interference between Human IGFBP2 and analogues was observed.
Sequence Length
328
Samples
Serum, plasma, tissue homogenates
Assay Type
Sandwich
Detection Range
0.78 ng/ml-50 ng/ml
Sensitivity
0.195 ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Protein Biological Process 1
Growth Factor
Protein Biological Process 3
Growth regulation
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for IGFBP2 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IGFBP-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IGFBP-2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IGFBP-2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IGFBP-2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
34,814 Da
NCBI Official Full Name
insulin-like growth factor-binding protein 2
NCBI Official Synonym Full Names
insulin-like growth factor binding protein 2, 36kDa
NCBI Official Symbol
IGFBP2
NCBI Official Synonym Symbols
IBP2; IGF-BP53
NCBI Protein Information
insulin-like growth factor-binding protein 2; IBP-2; IGFBP-2; IGF-binding protein 2
UniProt Protein Name
Insulin-like growth factor-binding protein 2
Protein Family
UniProt Gene Name
IGFBP2
UniProt Synonym Gene Names
BP2; IBP2; IBP-2; IGF-binding protein 2; IGFBP-2
UniProt Entry Name
IBP2_HUMAN
Uniprot Description
Function: Inhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.1 PublicationManual assertion based on experiment in:Ref.11
Research Articles on IGFBP2
1. High IGFBP-2 gene expression is asssociated with cytomegalovirus positive glioblastoma multiforme.
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Product Notes
The Human IGFBP2 igfbp2 (Catalog #AAA700515) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA700515 ELISA Kit recognizes Human IGFBP2. It is sometimes possible for the material contained within the vial of "insulin-like growth factor binding protein 2, 36kDa, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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