Testing Data
RBC Reagent
RBC Lysis Buffer (10X)
Synonyms
RBC; RBC Lysis Buffer (10X); RBC reagent
Form/Format
Liquid
Application Notes
The processed blood or tissue cell samples can be used for subsequent primary culture, cell fusion, nucleic acid or protein extraction, and various conventional analysis and detection.
Procedure for Lysis of Tissue Cells
RBC Lysis Buffer (10X) should be diluted in deionized water prior to use.
1. Digest fresh tissue samples and prepare a single-cell suspension.
2. Centrifuge harvested cell suspension at 400-500xg for 5min at 4 degree C, then carefully remove and discard the supernatant.
3. Add 1X prepared RBC Lysis Buffer to the cell pellet at 3:1 to 5:1 (i.e. Add 3-5mL 1X prepared RBC Lysis Buffer to 1mL cell pellet.) Vortex briefly. Incubate on ice for 4-5 min. Shake gently during incubation.
4. Centrifuge at 400-500xg for 5min at 4 degree C, then carefully remove and discard the red supernatant.
5. (Optional) Repeat Step 4 and Step 5 if further removal of red blood cells is needed. Small numbers of residual red blood cells do not interfere with subsequent assays.
6. Resuspend the cell pellet in at least 5 volume PBS, HBSS, normal saline or serum free medium for 1-2 times. Centrifuge at 400-500xg for 2-3 min at 4 degree C, then carefully remove and discard the supernatant.
7. Count the cells, adjust the density, and stain the cells.
1. Digest fresh tissue samples and prepare a single-cell suspension.
2. Centrifuge harvested cell suspension at 400-500xg for 5min at 4 degree C, then carefully remove and discard the supernatant.
3. Add 1X prepared RBC Lysis Buffer to the cell pellet at 3:1 to 5:1 (i.e. Add 3-5mL 1X prepared RBC Lysis Buffer to 1mL cell pellet.) Vortex briefly. Incubate on ice for 4-5 min. Shake gently during incubation.
4. Centrifuge at 400-500xg for 5min at 4 degree C, then carefully remove and discard the red supernatant.
5. (Optional) Repeat Step 4 and Step 5 if further removal of red blood cells is needed. Small numbers of residual red blood cells do not interfere with subsequent assays.
6. Resuspend the cell pellet in at least 5 volume PBS, HBSS, normal saline or serum free medium for 1-2 times. Centrifuge at 400-500xg for 2-3 min at 4 degree C, then carefully remove and discard the supernatant.
7. Count the cells, adjust the density, and stain the cells.
Procedure for Lysis of Whole Blood
RBC Lysis Buffer (10X) should be diluted in deionized water prior to use.
1. Centrifuge harvested fresh anticoagulant blood at 400-500xg for 5min at 4 degree C, then carefully remove and discard the supernatant.
2. Add 1X prepared RBC Lysis Buffer to the cell pellet at 10:1 (i.e. Add 10mL 1X prepared RBC Lysis Buffer to 1mL cell pellet.) Vortex briefly. Incubate on ice for 4-5 min. Shake gently during incubation.
3. Centrifuge at 400-500xg for 5min at 4 degree C, then carefully remove and discard the red supernatant.
4. (Optional) Repeat Step 2 and Step 3 if further removal of red blood cells is needed. Small numbers of residual red blood cells do not interfere with subsequent assays.
5. Resuspend the cell pellet in at least 5 volume PBS, HBSS, normal saline or serum free medium for 1-2 times. Centrifuge at 400-500xg for 2-3 min at 4 degree C, then carefully remove and discard the supernatant.
6. Count the cells, adjust the density, and stain the cells.
Notes: For a small number of blood cells, Step 1 can be omitted. In Step 2, for mouse blood, it is sufficient to incubate on ice for 4-5 min. For human peripheral blood, it is better to extend the incubation time to 10 min, but no longer than 15 min. Shake gently during incubation. Step 4 to Step 6 remain the same.
1. Centrifuge harvested fresh anticoagulant blood at 400-500xg for 5min at 4 degree C, then carefully remove and discard the supernatant.
2. Add 1X prepared RBC Lysis Buffer to the cell pellet at 10:1 (i.e. Add 10mL 1X prepared RBC Lysis Buffer to 1mL cell pellet.) Vortex briefly. Incubate on ice for 4-5 min. Shake gently during incubation.
3. Centrifuge at 400-500xg for 5min at 4 degree C, then carefully remove and discard the red supernatant.
4. (Optional) Repeat Step 2 and Step 3 if further removal of red blood cells is needed. Small numbers of residual red blood cells do not interfere with subsequent assays.
5. Resuspend the cell pellet in at least 5 volume PBS, HBSS, normal saline or serum free medium for 1-2 times. Centrifuge at 400-500xg for 2-3 min at 4 degree C, then carefully remove and discard the supernatant.
6. Count the cells, adjust the density, and stain the cells.
Notes: For a small number of blood cells, Step 1 can be omitted. In Step 2, for mouse blood, it is sufficient to incubate on ice for 4-5 min. For human peripheral blood, it is better to extend the incubation time to 10 min, but no longer than 15 min. Shake gently during incubation. Step 4 to Step 6 remain the same.
Preparation and Storage
Upon receipt store at 4 degree C. RBC Lysis Buffer (10X) is stable for one year. Product is shipped on ice.
Testing Data
Testing Data
Related Product Information for RBC reagent
RBC Lysis Buffer is supplied as a 10X solution contains ammonium chloride, which lyses red blood cells with minimal effects on leukocytes or other cells with nucleus.
RBC (Red Blood Cell) Lysis Buffer has been designed, formulated, and tested to ensure optimal lysis of RBCs in single cell suspensions of blood or tissue samples from human or mouse. RBC Lysis Buffer is supplied as a 10X solution contains ammonium chloride, which lyses red blood cells with minimal effects on leukocytes or other cells with nucleus. Nucleated RBCs are not effectively lysed with ammonium chloride. The buffer should be diluted in deionized water prior to use. It is sterile filtered, and the processed blood or tissue cell samples can be used for subsequent primary culture, cell fusion, nucleic acid or protein extraction, and various conventional analysis and detection.
RBC (Red Blood Cell) Lysis Buffer has been designed, formulated, and tested to ensure optimal lysis of RBCs in single cell suspensions of blood or tissue samples from human or mouse. RBC Lysis Buffer is supplied as a 10X solution contains ammonium chloride, which lyses red blood cells with minimal effects on leukocytes or other cells with nucleus. Nucleated RBCs are not effectively lysed with ammonium chloride. The buffer should be diluted in deionized water prior to use. It is sterile filtered, and the processed blood or tissue cell samples can be used for subsequent primary culture, cell fusion, nucleic acid or protein extraction, and various conventional analysis and detection.
Product Categories/Family for RBC reagent
Similar Products
Product Notes
The RBC (Catalog #AAA1753882) is a Reagent and is intended for research purposes only. The product is available for immediate purchase. The processed blood or tissue cell samples can be used for subsequent primary culture, cell fusion, nucleic acid or protein extraction, and various conventional analysis and detection. Researchers should empirically determine the suitability of the RBC for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RBC, Reagent" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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