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Typical Testing Data/Standard Curve (for reference only)

Mouse gelsolin (amyloidosis, Finnish type) ELISA Kit | GSN elisa kit

Mouse Gelsolin ELISA Kit

Gene Names
Gsn; ADF
Reactivity
Mouse
Synonyms
gelsolin (amyloidosis; Finnish type); Mouse Gelsolin ELISA Kit; RP11-477J21.1; DKFZp313L0718; OTTHUMP00000162202; gelsolin; GSN elisa kit
Ordering
For Research Use Only!
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse GSN. No significant cross-reactivity or interference between Mouse GSN and analogues was observed.
Sequence Length
780
Samples
Serum, plasma, cell culture supernates, tissue homogenates
Assay Type
Sandwich
Detection Range
15.6 ng/ml-1000 ng/ml
Sensitivity
3.9 ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Protein Biological Process 1
Biosynthesis/Metabolism
Protein Biological Process 2
Amino-acid biosynthesis and metabolism
Protein Biological Process 3
Cilium biogenesis/degradation
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for GSN elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for Gelsolin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Gelsolin present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Gelsolin is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Gelsolin bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
85,942 Da
NCBI Official Full Name
gelsolin isoform 1
NCBI Official Synonym Full Names
gelsolin
NCBI Official Symbol
Gsn
NCBI Official Synonym Symbols
ADF
NCBI Protein Information
gelsolin; brevin; actin-depolymerizing factor
UniProt Protein Name
Gelsolin
UniProt Gene Name
Gsn
UniProt Synonym Gene Names
Gsb; ADF
UniProt Entry Name
GELS_MOUSE

Uniprot Description

Function: Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis.

Research Articles on GSN

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Product Notes

The Mouse GSN gsn (Catalog #AAA702170) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA702170 ELISA Kit recognizes Mouse GSN. It is sometimes possible for the material contained within the vial of "gelsolin (amyloidosis, Finnish type), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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