Typical Testing Data/Standard Curve (for reference only)
Mouse Neurotensin Receptor 1, High Affinity (NTSR1) ELISA Kit | NTSR1 elisa kit
Mouse Neurotensin Receptor 1, High Affinity (NTSR1) ELISA Kit
Gene Names
NTSR1; NTR
Reactivity
Mouse
Synonyms
Neurotensin Receptor 1; High Affinity (NTSR1); Mouse Neurotensin Receptor 1; High Affinity (NTSR1) ELISA Kit; NTR; NTRH; NTRR; High-affinity levocabastine-insensitive neurotensin receptor; NTSR1 elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Neurotensin Receptor 1, High Affinity (NTSR1).
No significant cross-reactivity or interference between Neurotensin Receptor 1, High Affinity (NTSR1) and analogues was observed.
No significant cross-reactivity or interference between Neurotensin Receptor 1, High Affinity (NTSR1) and analogues was observed.
Sequence Length
418
Assay Type
Double-antibody Sandwich
Samples
Tissue homogenates and Other Biological Fluids
Detection Range
0.156-10ng/mL
Sensitivity
< 0.066ng/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Neurotensin Receptor 1, High Affinity (NTSR1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Neurotensin Receptor 1, High Affinity (NTSR1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for NTSR1 elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neurotensin Receptor 1, High Affinity (NTSR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neurotensin Receptor 1, High Affinity (NTSR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neurotensin Receptor 1, High Affinity (NTSR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neurotensin Receptor 1, High Affinity (NTSR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for NTSR1 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
46,259 Da
NCBI Official Full Name
neurotensin receptor type 1
NCBI Official Synonym Full Names
neurotensin receptor 1 (high affinity)
NCBI Official Symbol
NTSR1
NCBI Official Synonym Symbols
NTR
NCBI Protein Information
neurotensin receptor type 1
UniProt Protein Name
Neurotensin receptor type 1
Protein Family
UniProt Gene Name
NTSR1
UniProt Synonym Gene Names
NTRR; NT-R-1; NTR1
UniProt Entry Name
NTR1_HUMAN
NCBI Description
Neurotensin receptor 1 belongs to the large superfamily of G-protein coupled receptors. NTSR1 mediates the multiple functions of neurotensin, such as hypotension, hyperglycemia, hypothermia, antinociception, and regulation of intestinal motility and secretion. [provided by RefSeq, Jul 2008]
Uniprot Description
NTSR1: Receptor for the tridecapeptide neurotensin. It is associated with G proteins that activate a phosphatidylinositol- calcium second messenger system. Belongs to the G-protein coupled receptor 1 family. Neurotensin receptor subfamily. NTSR1 sub-subfamily.
Protein type: Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR; GPCR, family 1
Chromosomal Location of Human Ortholog: 20q13
Cellular Component: Golgi apparatus; endoplasmic reticulum; integral to plasma membrane; plasma membrane; lipid raft
Molecular Function: G-protein coupled receptor activity; neurotensin receptor activity, G-protein coupled; protein binding
Biological Process: synaptic transmission; G-protein coupled receptor protein signaling pathway; neuropeptide signaling pathway; adult locomotory behavior; negative regulation of apoptosis
Research Articles on NTSR1
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Product Notes
The Mouse NTSR1 ntsr1 (Catalog #AAA2000015) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2000015 ELISA Kit recognizes Mouse NTSR1. It is sometimes possible for the material contained within the vial of "Neurotensin Receptor 1, High Affinity (NTSR1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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