Typical Testing Data/Standard Curve (for reference only)
Mouse chemokine (C-X-C motif) ligand 10 ELISA Kit | CXCL10 elisa kit
Mouse interferon-inducible protein 10, IP-10 ELISA Kit
Gene Names
Cxcl10; C7; IP10; CRG-2; INP10; IP-10; Ifi10; mob-1; Scyb10; gIP-10
Reactivity
Mouse
Synonyms
chemokine (C-X-C motif) ligand 10; Mouse interferon-inducible protein 10; IP-10 ELISA Kit; C7; IFI10; INP10; IP-10; SCYB10; crg-2; gIP-10; mob-1; gamma IP10; interferon-inducible cytokine IP-10; protein 10 from interferon (gamma) -induced cell line; small inducible cytokine B10; small inducibl; CXCL10 elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse CXCL10. No significant cross-reactivity or interference between Mouse CXCL10 and analogues was observed.
Sequence Length
98
Detection Range
3.2 pg/ml-200 pg/ml
Sensitivity
0.8 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Protein Biological Process 1
Immunity
Protein Biological Process 2
chemokine
Protein Biological Process 3
Chemotaxis
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CXCL10 elisa kit
Introduction: Interferon-inducible protein-10 (IP-10) is a member of the C-X-C chemokine family that is also known as 10 kDa interferon-gamma-induced protein (gamma-IP10 or IP-10). IP-10 is secreted by several cell types in response to IFN-gamma. These cell types include monocytes, endothelial cells and fibroblasts. IP-10 has been attributed to several roles, such as chemoattraction for monocytes/macrophages, T cells, NK cells, and dendritic cells, promotion of T cell adhesion to endothelial cells, antitumor activity, and inhibition of bone marrow colony formation and angiogenesis. The gene for IP-10 is located on human chromosome 4 in a cluster among several other CXC chemokines. This chemokine elicits its effects by binding to the cell surface chemokine receptor CXCR3. The three-dimensional crystal structure of this chemokine has been determined under 3 different conditions to a resolution of up to 1.92A. The Protein Data Bank accession codes for the structures of CXCL10 are 1lv9, 1o7y, 1o7z and 1o80.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IP-10. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IP-10 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain IP-10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of IP-10 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IP-10. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IP-10 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain IP-10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of IP-10 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
10,789 Da
NCBI Official Full Name
C-X-C motif chemokine 10
NCBI Official Synonym Full Names
chemokine (C-X-C motif) ligand 10
NCBI Official Symbol
Cxcl10
NCBI Official Synonym Symbols
C7; IP10; CRG-2; INP10; IP-10; Ifi10; mob-1; Scyb10; gIP-10
NCBI Protein Information
C-X-C motif chemokine 10; gamma-IP10; interferon activated gene 10; small-inducible cytokine B10; interferon-gamma induced protein CRG-2; interferon-gamma-induced protein CRG-2; 10 kDa interferon gamma-induced protein; small inducible cytokine B subfamily (Cys-X-Cys), member 10
UniProt Protein Name
C-X-C motif chemokine 10
Protein Family
UniProt Gene Name
Cxcl10
UniProt Synonym Gene Names
Crg2; Ifi10; Inp10; Scyb10; Gamma-IP10; IP-10
UniProt Entry Name
CXL10_MOUSE
Uniprot Description
Function: In addition to its role as a proinflammatory cytokine, may participate in T-cell effector function and perhaps T-cell development.
Research Articles on CXCL10
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Product Notes
The Mouse CXCL10 cxcl10 (Catalog #AAA701931) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA701931 ELISA Kit recognizes Mouse CXCL10. It is sometimes possible for the material contained within the vial of "chemokine (C-X-C motif) ligand 10, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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