Mouse CXCL10/IP-10 ELISA Kit | CXCL10 elisa kit

Mouse CXCL10/IP-10 PicoKine ELISA Kit

Cat. Num. AAA175884

• Gene Names: Cxcl10; C7; IP10; CRG-2; INP10; IP-10; Ifi10; mob-1; Scyb10; gIP-10
• Reactivity: Mouse
• Synonyms: CXCL10/IP-10; Mouse CXCL10/IP-10 PicoKine ELISA Kit; C-X-C motif chemokine 10; chemokine (C-X-C motif) ligand 10; 10 kDa interferon gamma-induced protein; C7; Chemokine (C X C motif) ligand 10; Chemokine CXC motif ligand 10; Crg 2; CRG2; CXCL10; CXCL10(1-73); CXL10_HUMAN; Gamma IP10; Gamma-IP10; gIP 10; GIP10; IFI10; INP 10; Interferon activated gene 10; Interferon gamma induced cell line; Interferon inducible cytokine IP10; IP 10; IP-10; MOB1; SCYB10; Small inducible cytokine B10; Small inducible cytokine B10 precursor; Small inducible cytokine subfamily B (Cys X Cys) member 10; Small inducible cytokine subfamily B CXC member 10; Small-inducible cytokine B10; CXCL10 elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant mouse CXCL10
• Sequence Length: 98

• Samples: Cell culture supernates, serum and plasma (heparin)
• Assay Type: Sandwich
• Detection Range: 31.2pg/ml-2000pg/ml
• Sensitivity: < 1pg/ml
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Mouse CXCL10/IP-10 PicoKine ELISA Kit standard curve )

Related Product Information for CXCL10 elisa kit

Principle of the assay: mouse CXCL10 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CXCL10 has been precoated onto 96-well plates. Standards(E.coli,I22-P98) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL10 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CXCL10 amount of sample captured in plate.
Background: Chemokine (C-X-C motif) ligand 10 (CXCL10) or IP-10 is a small cytokine belonging to the CXC chemokine family that is also known as 10 kDa interferon-gamma-induced protein (gamma-IP10 or IP-10). CXCL10 is secreted by several cell types in response to IFN-gamma. These cell types include monocytes, endothelial cells and fibroblasts. CXCL10 has been attributed to several roles, such as chemoattraction for monocytes/macrophages, T cells, NK cells, and dendritic cells, promotion of T cell adhesion to endothelial cells, antitumor activity, and inhibition of bone marrow colony formation and angiogenesis.It is a potent inhibitor of angiogenesis in vivo. The gene for CXCL10 is located on human chromosome 4 in a cluster among several other CXC chemokines. It is a RAS target gene and is overexpressed in the majority of colorectal cancers.This chemokine elicits its effects by binding to the cell surface chemokine receptor CXCR3.

NCBI and Uniprot Product Information

• NCBI GI #: 124494
• NCBI GeneID: 15945
• UniProt Accession #: P17515
• Molecular Weight: 10,789 Da
• NCBI Official Full Name: C-X-C motif chemokine 10
• NCBI Official Synonym Full Names: chemokine (C-X-C motif) ligand 10
• NCBI Official Symbol: Cxcl10
• NCBI Official Synonym Symbols: C7; IP10; CRG-2; INP10; IP-10; Ifi10; mob-1; Scyb10; gIP-10
• NCBI Protein Information: C-X-C motif chemokine 10; gamma-IP10; interferon activated gene 10; small-inducible cytokine B10; interferon-gamma induced protein CRG-2; interferon-gamma-induced protein CRG-2; 10 kDa interferon gamma-induced protein; small inducible cytokine B subfamily (Cys-X-Cys), member 10
• UniProt Protein Name: C-X-C motif chemokine 10
• Protein Family: C-X-C motif chemokine
• UniProt Gene Name: Cxcl10
• UniProt Synonym Gene Names: Crg2; Ifi10; Inp10; Scyb10; Gamma-IP10; IP-10
• UniProt Entry Name: CXL10_MOUSE

Uniprot Description

CXCL10: Chemotactic for monocytes and T-lymphocytes. Binds to CXCR3. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Motility/polarity/chemotaxis; Chemokine; Secreted, signal peptide; Secreted

Cellular Component: extracellular space; cell; extracellular region; external side of plasma membrane

Molecular Function: heparin binding; CXCR3 chemokine receptor binding; chemokine activity; cytokine activity

Biological Process: positive regulation of leukocyte chemotaxis; response to lipopolysaccharide; positive regulation of cAMP metabolic process; chemotaxis; regulation of cell proliferation; G-protein coupled receptor protein signaling pathway; negative regulation of angiogenesis; positive regulation of cell proliferation; immune response; positive regulation of release of sequestered calcium ion into cytosol; negative regulation of myoblast differentiation; inflammatory response; defense response to virus; positive regulation of cell migration