Western Blot (WB)
(Western Blot analysis in A549 cell line lysates (35ug/lane) using MBS6003454. This demonstrates that MBS6003454 detected the NEU2 protein (arrow).)
Rabbit anti-Human NEU2 Polyclonal Antibody | anti-NEU2 antibody
NEU2, NT (NEU2, Sialidase-2, Cytosolic sialidase, N-acetyl-alpha-neuraminidase 2)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50
Immunofluorescence: 1:10-50
Flow Cytometry: 1:10-50
Western Blot (WB)
(Western Blot analysis in A549 cell line lysates (35ug/lane) using MBS6003454. This demonstrates that MBS6003454 detected the NEU2 protein (arrow).)
Western Blot (WB)
(Western Blot analysis in A549 cell line lysates (35ug/lane) using MBS6003454. This demonstrates that MBS6003454 detected the NEU2 protein (arrow).)
Western Blot (WB)
(Western Blot analysis in human heart tissue lysate (20ug) using MBS6003454 (1:2000). Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000. Predicted band size: 42kD. Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Western Blot analysis in human heart tissue lysate (20ug) using MBS6003454 (1:2000). Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000. Predicted band size: 42kD. Blocking/Dilution buffer: 5% NFDM/TBST.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue using MBS6003454 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6003454 for immunohistochemistry.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue using MBS6003454 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6003454 for immunohistochemistry.)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis with A549 cells using MBS6003454 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis with A549 cells using MBS6003454 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)
NCBI and Uniprot Product Information
NCBI Description
This gene belongs to a family of glycohydrolytic enzymes which remove sialic acid residues from glycoproteins and glycolipids. Expression studies in COS7 cells confirmed that this gene encodes a functional sialidase. Its cytosolic localization was demonstrated by cell fractionation experiments. [provided by RefSeq, Jul 2008]
Uniprot Description
NEU2: Hydrolyzes sialylated compounds. Belongs to the glycosyl hydrolase 33 family.
Protein type: EC 3.2.1.18; Glycan Metabolism - other glycan degradation; Hydrolase; Lipid Metabolism - sphingolipid
Chromosomal Location of Human Ortholog: 2q37
Cellular Component: cytoplasm; cytosol
Biological Process: oligosaccharide catabolic process; cellular protein metabolic process; sphingolipid metabolic process; dolichol-linked oligosaccharide biosynthetic process; ganglioside catabolic process; protein amino acid N-linked glycosylation via asparagine; glycosphingolipid metabolic process; post-translational protein modification
Research Articles on NEU2
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Product Notes
The NEU2 neu2 (Catalog #AAA6003454) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NEU2, NT (NEU2, Sialidase-2, Cytosolic sialidase, N-acetyl-alpha-neuraminidase 2) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NEU2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF). Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:10-50 Immunofluorescence: 1:10-50 Flow Cytometry: 1:10-50. Researchers should empirically determine the suitability of the NEU2 neu2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NEU2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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