Sheep interleukin 2 ELISA Kit | IL2 elisa kit

Sheep Interleukin 2, IL-2 ELISA Kit

Cat. Num. AAA701196

• Gene Names: IL2; IL-2
• Reactivity: Sheep
• Synonyms: interleukin 2; Sheep Interleukin 2; IL-2 ELISA Kit; IL-2; TCGF; lymphokine; T cell growth factor; aldesleukin; interleukin-2; involved in regulation of T-cell clonal expansion; IL2 elisa kit

• Reactivity: Sheep
• Specificity: This assay has high sensitivity and excellent specificity for detection of Sheep IL2. No significant cross-reactivity or interference between Sheep IL2 and analogues was observed.
• Sequence Length: 155

• Samples: Serum, plasma, tissue homogenates
• Detection Range: 31.25 pg/ml-2000 pg/ml
• Sensitivity: 7.8 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Immunity
• Protein Biological Process 3: Adaptive immunity
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL2 elisa kit

Introduction: Interleukin-2 (IL-2) is an interleukin, a type of cytokine immune system signaling molecule, that is instrumental in the body's natural response to microbial infection and in discriminating between foreign (non-self) and self. IL-2 mediates its effects by binding to IL-2 receptors, which are expressed by lymphocytes, the cells that are responsible for immunity. IL-2 signals through a receptor complex consisting of IL-2 specific IL-2 receptor alpha (CD25), IL-2 receptor beta (CD122) and a common gamma chain (gammac), which is shared by all members of this family of cytokines. Binding of IL-2 activates the Ras/MAPK, JAK/Stat and PI 3-kinase/Akt signaling modules. IL-2 is normally produced by the body during an immune response. When environmental substances (molecules or microbes) gain access to the body, these substances (termed antigens) are recognized as foreign by antigen receptors that are expressed on the surface of lymphocytes. Antigen binding to the T cell receptor (TCR) stimulates the secretion of IL-2, and the expression of IL-2 receptors IL-2R. The IL-2/IL-2R interaction then stimulates the growth, differentiation and survival of antigen-selected cytotoxic T cells via the activation of the expression of specific genes. As such, IL-2 is necessary for the development of T cell immunologic 3 memory. IL-2 is also necessary during T cell development in the thymus for the maturation of a unique subset of T cells that are termed regulatory T cells (T-regs). After exiting from the thymus, T-Regs function to prevent other T cells from recognizing and reacting against "self antigens", which could result in "autoimmunity". T-Regs do so by preventing the responding cells from producing IL-2. Thus, IL-2 is required to discriminate between self and non-self, another one of the unique characteristics of the immune system. IL-2 has been found to be similar to IL-15 in terms of function. Both cytokines are able to facilitate production of immunoglobulins made by B cells and induce the differentiation and proliferation of natural killer cells. The primary differences between IL-2 and IL-15 are found in adaptive immune responses. For example, IL-2 participates in maintenance of T-Regs and reduces self-reactive T cells. On the other hand, IL-15 is necessary for maintaining highly specific T cell responses by supporting survival of CD8 memory T cells. The differences in function in these two cytokines stem from the signal transduction mechanisms and differing receptors.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for IL-2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain IL-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of IL-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 57526778
• NCBI GeneID: 443401
• NCBI Accession #: NP_001009806.1
• NCBI GenBank Nucleotide #: NM_001009806.1
• UniProt Accession #: P19114; Q95MP4; A0MZP0
• Molecular Weight: 17,678 Da
• NCBI Official Full Name: interleukin-2
• NCBI Official Symbol: IL2
• NCBI Official Synonym Symbols: IL-2
• NCBI Protein Information: interleukin-2; TCGF; T-cell growth factor
• UniProt Protein Name: Interleukin-2
• Protein Family: Interleukin
• UniProt Gene Name: IL2
• UniProt Synonym Gene Names: IL-2; TCGF
• UniProt Entry Name: IL2_SHEEP

Uniprot Description

Function: Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.