Western Blot (WB)
(Western blot analysis of anti-Ubiquitin Pab in HeLa cell lysate. Ubiquitin (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
Rabbit Ubiquitin Polyclonal Antibody | anti-UBC antibody
Ubiquitin Antibody (C-term)
Western Blot (WB)
(Western blot analysis of anti-Ubiquitin Pab in HeLa cell lysate. Ubiquitin (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
Western Blot (WB)
(Western blot analysis of anti-Ubiquitin Pab in HeLa cell lysate. Ubiquitin (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Immunofluorescence (IF)
(Characterization of FOXP2 Isoforms. FOXP2.10t was detected with an antibody to the N-terminal XpressTM tag or FOXP2 (green) and counterstained with antibodies to the aggresome marker ubiquitin (red). Nuclei are marked by DAPI staining (blue). Ubiquitin co-localizes with FOXP2.10t aggregates suggesting that these cellular bodies represent aggresomes. (Hum. Mol. Genet. 2006 Nov 01;15(21):3154-3167))
Immunofluorescence (IF)
(Characterization of FOXP2 Isoforms. FOXP2.10t was detected with an antibody to the N-terminal XpressTM tag or FOXP2 (green) and counterstained with antibodies to the aggresome marker ubiquitin (red). Nuclei are marked by DAPI staining (blue). Ubiquitin co-localizes with FOXP2.10t aggregates suggesting that these cellular bodies represent aggresomes. (Hum. Mol. Genet. 2006 Nov 01;15(21):3154-3167))
Cook, W.J., et al., J. Mol. Biol. 236(2):601-609 (1994).
Hubbard, M.J., et al., Biochim. Biophys. Acta 1200(2):191-196 (1994).
Wajih, N., et al., Protein Seq. Data Anal. 5(1):31-32 (1992).
Cook, W.J., et al., J. Biol. Chem. 267(23):16467-16471 (1992).
NCBI and Uniprot Product Information
NCBI Description
This gene represents a ubiquitin gene, ubiquitin C. The encoded protein is a polyubiquitin precursor. Conjugation of ubiquitin monomers or polymers can lead to various effects within a cell, depending on the residues to which ubiquitin is conjugated. Ubiquitination has been associated with protein degradation, DNA repair, cell cycle regulation, kinase modification, endocytosis, and regulation of other cell signaling pathways. [provided by RefSeq, Aug 2010]
Uniprot Description
UBC: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. Belongs to the ubiquitin family.
Protein type: Ubiquitin-like modifier
Chromosomal Location of Human Ortholog: 12q24.3
Cellular Component: nucleoplasm; plasma membrane; endosome membrane; cytosol
Molecular Function: protein binding; protease binding
Biological Process: circadian rhythm; I-kappaB kinase/NF-kappaB cascade; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; nerve growth factor receptor signaling pathway; activation of MAPK activity; positive regulation of apoptosis; stress-activated MAPK cascade; toll-like receptor 3 signaling pathway; endosome transport; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; T cell receptor signaling pathway; activation of NF-kappaB transcription factor; regulation of apoptosis; toll-like receptor 5 signaling pathway; antigen processing and presentation of peptide antigen via MHC class I; transforming growth factor beta receptor signaling pathway; JNK cascade; antigen processing and presentation of exogenous peptide antigen via MHC class I; G2/M transition of mitotic cell cycle; toll-like receptor 4 signaling pathway; regulation of interferon type I production; glycogen biosynthetic process; fibroblast growth factor receptor signaling pathway; positive regulation of I-kappaB kinase/NF-kappaB cascade; transcription, DNA-dependent; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; Notch receptor processing; glucose metabolic process; virus assembly; toll-like receptor 2 signaling pathway; carbohydrate metabolic process; viral protein processing; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; negative regulation of interferon type I production; negative regulation of apoptosis; G1/S transition of mitotic cell cycle; negative regulation of epidermal growth factor receptor signaling pathway; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; apoptosis; pathogenesis; viral infectious cycle; negative regulation of transcription from RNA polymerase II promoter; toll-like receptor 10 signaling pathway; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; positive regulation of interferon type I production; transmembrane transport; epidermal growth factor receptor signaling pathway; transcription initiation from RNA polymerase II promoter; Notch signaling pathway; MyD88-independent toll-like receptor signaling pathway; cytokine and chemokine mediated signaling pathway; DNA repair; MyD88-dependent toll-like receptor signaling pathway; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; toll-like receptor signaling pathway; innate immune response; gene expression; mitotic cell cycle; negative regulation of transforming growth factor beta receptor signaling pathway
Research Articles on UBC
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Product Notes
The UBC ubc (Catalog #AAA9203298) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 36-66. The Ubiquitin Antibody (C-term) reacts with Human (Predicted Reactivity: Yeast, Bovine, C Elegans, Drosophila, Monkey, Mouse, Pig, Rat, Chicken, Hamster, Xenopus, Rabbit) and may cross-react with other species as described in the data sheet. AAA Biotech's Ubiquitin can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the UBC ubc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ubiquitin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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