Mouse apelin ELISA Kit | APLN elisa kit

mouse Apelin ELISA Kit

Cat. Num. AAA701195

• Gene Names: Apln; Apel; 6030430G11Rik
• Reactivity: Mouse
• Synonyms: apelin; mouse Apelin ELISA Kit; mouse Apelin ELISA kit; XNPEP2; AGTRL1 ligand; peptide ligand for APJ receptor; APLN elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse apelin. No significant cross-reactivity or interference between mouse apelin and analogues was observed.
• Sequence Length: 77

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 31.25 pg/ml-2000 pg/ml
• Sensitivity: < 7.81 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for APLN elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for apelin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any apelin present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for apelin is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of apelin bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 7304895
• NCBI GeneID: 30878
• NCBI Accession #: NP_038940.1
• NCBI GenBank Nucleotide #: NM_013912.3
• UniProt Accession #: Q9R0R4
• Molecular Weight: 8,658 Da
• NCBI Official Full Name: apelin
• NCBI Official Synonym Full Names: apelin
• NCBI Official Symbol: Apln
• NCBI Official Synonym Symbols: Apel; 6030430G11Rik
• NCBI Protein Information: apelin; preproapelin; APJ endogenous ligand
• UniProt Protein Name: Apelin
• Protein Family: Apelin
• UniProt Gene Name: Apln
• UniProt Synonym Gene Names: Apel
• UniProt Entry Name: APEL_MOUSE

Uniprot Description

Function: Endogenous ligand for APJ. Has an inhibitory effect on cytokine production in response to T-cell receptor/CD3 cross-linking. The oral intake of apelin in the colostrum and the milk might therefore modulate immune responses in neonates. May also have a role in the central control of body fluid homeostasis by influencing AVP release and drinking behavior.