Typical Testing Data/Standard Curve (for reference only)
Human secreted frizzled-related protein 4 ELISA Kit | SFRP4 elisa kit
Human secreted frizzled-related protein 4, SFRP4 ELISA Kit
Gene Names
SFRP4; FRP-4; FRPHE; sFRP-4
Reactivity
Human
Synonyms
secreted frizzled-related protein 4; Human secreted frizzled-related protein 4; SFRP4 ELISA Kit; Human secreted frizzled-related protein 4 (SFRP4) ELISA Kit; FRP-4; FRPHE; MGC26498; SFRP4 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human SFRP4. No significant cross-reactivity or interference between Human SFRP4 and analogues was observed.
Sequence Length
346
Samples
Serum, plasma, tissue homogenates
Assay Type
Sandwich
Detection Range
1.56 ng/ml-100ng/ml
Sensitivity
0.39 ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Protein Biological Process 1
Developmental Protein
Protein Biological Process 3
Differentiation
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for SFRP4 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SFRP4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SFRP4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SFRP4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SFRP4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
39,827 Da
NCBI Official Full Name
secreted frizzled-related protein 4
NCBI Official Synonym Full Names
secreted frizzled-related protein 4
NCBI Official Symbol
SFRP4
NCBI Official Synonym Symbols
FRP-4; FRPHE; sFRP-4
NCBI Protein Information
secreted frizzled-related protein 4; frizzled protein, human endometrium; secreted frizzled-related protein 4; secreted frizzled-related protein 4
UniProt Protein Name
Secreted frizzled-related protein 4
Protein Family
UniProt Gene Name
SFRP4
UniProt Synonym Gene Names
FRPHE; sFRP-4; FrpHE
UniProt Entry Name
SFRP4_HUMAN
NCBI Description
Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. The expression of SFRP4 in ventricular myocardium correlates with apoptosis related gene expression. [provided by RefSeq, Jul 2008]
Uniprot Description
Function: Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP4 may act as a regulator of adult uterine morphology and function. Increases apoptosis during ovulation possibly through modulation of FZ1/FZ4/WNT4 signaling Has phosphaturic effects by specifically inhibiting sodium-dependent phosphate uptake.1 PublicationManual assertion based on experiment in:Ref.2
Research Articles on SFRP4
Similar Products
Product Notes
The Human SFRP4 sfrp4 (Catalog #AAA700680) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA700680 ELISA Kit recognizes Human SFRP4. It is sometimes possible for the material contained within the vial of "secreted frizzled-related protein 4, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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