Rat Secreted Frizzled Related Protein 4 (SFRP4) ELISA Kit | SFRP4 elisa kit

Rat Secreted Frizzled Related Protein 4 (SFRP4) ELISA Kit

Cat. Num. AAA2021277

• Gene Names: SFRP4; FRP-4; FRPHE; sFRP-4
• Reactivity: Rat
• Synonyms: Secreted Frizzled Related Protein 4 (SFRP4); Rat Secreted Frizzled Related Protein 4 (SFRP4) ELISA Kit; FRP-4; FRPHE; Frizzled protein; human endometrium; SFRP4 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Secreted Frizzled Related Protein 4 (SFRP4).; No significant cross-reactivity or interference between Secreted Frizzled Related Protein 4 (SFRP4) and analogues was observed.
• Sequence Length: 346

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 31.25-2000pg/mL
• Sensitivity: < 13.8pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Secreted Frizzled Related Protein 4 (SFRP4) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Secreted Frizzled Related Protein 4 (SFRP4) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve

Related Product Information for SFRP4 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Secreted Frizzled Related Protein 4 (SFRP4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Secreted Frizzled Related Protein 4 (SFRP4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Secreted Frizzled Related Protein 4 (SFRP4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Secreted Frizzled Related Protein 4 (SFRP4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for SFRP4 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 170784838
• NCBI GeneID: 6424
• NCBI Accession #: NP_003005.2
• NCBI GenBank Nucleotide #: NM_003014.3
• UniProt Accession #: Q6FHJ7; O14877; Q05BG7; Q1ZYW2; Q4G124; Q6FHM0; Q6PD64; B4DYC1
• Molecular Weight: 39,827 Da
• NCBI Official Full Name: secreted frizzled-related protein 4
• NCBI Official Synonym Full Names: secreted frizzled-related protein 4
• NCBI Official Symbol: SFRP4
• NCBI Official Synonym Symbols: FRP-4; FRPHE; sFRP-4
• NCBI Protein Information: secreted frizzled-related protein 4
• UniProt Protein Name: Secreted frizzled-related protein 4
• Protein Family: Secreted frizzled-related protein
• UniProt Gene Name: SFRP4
• UniProt Synonym Gene Names: FRPHE; sFRP-4; FrpHE
• UniProt Entry Name: SFRP4_HUMAN

NCBI Description

Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. The expression of SFRP4 in ventricular myocardium correlates with apoptosis related gene expression. [provided by RefSeq, Jul 2008]

Uniprot Description

SFRP4: Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP4 may act as a regulator of adult uterine morphology and function. Increases apoptosis during ovulation possibly through modulation of FZ1/FZ4/WNT4 signaling. Has phosphaturic effects by specifically inhibiting sodium-dependent phosphate uptake. Belongs to the secreted frizzled-related protein (sFRP) family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 7p14.1

Cellular Component: extracellular space; cell surface; cytoplasm; integral to membrane; nucleus

Molecular Function: G-protein coupled receptor activity; Wnt-protein binding; Wnt receptor activity; protein binding

Biological Process: negative regulation of cell proliferation; positive regulation of apoptosis; multicellular organismal development; negative regulation of transcription factor activity; positive regulation of epidermal cell differentiation; positive regulation of receptor internalization; response to hormone stimulus; phosphate ion homeostasis; cell differentiation