Human Secreted frizzled-related protein 4 ELISA Kit | SFRP4 elisa kit

Human Secreted frizzled-related protein 4 ELISA Kit

Cat. Num. AAA764667

• Gene Names: SFRP4; FRP-4; FRPHE; sFRP-4
• Reactivity: Human
• Synonyms: Secreted frizzled-related protein 4; Human Secreted frizzled-related protein 4 ELISA Kit; SFRP4 (Secreted frizzled-related protein 4)/DDC-4/sFRP-4/FRP-4/FRP-AP/frpHE/FrzB2/Frizzled protein; human endometrium/secreted frizzled-related protein 4/secreted frizzled-related protein 4; SFRP4 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human SFRP4. No significant cross-reactivity or interference between Human SFRP4 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the crossreactivity detection between Human SFRP4 and all the analogues, therefore, cross reaction may still exist.
• Sequence Length: 346

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Human SFRP4 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Human SFRP4 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for SFRP4 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human SFRP4 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human SFRP4 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human SFRP4 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human SFRP4 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 170784838
• NCBI GeneID: 6424
• NCBI Accession #: NP_003005.2
• NCBI GenBank Nucleotide #: NM_003014.3
• Molecular Weight: 39,827 Da
• NCBI Official Full Name: secreted frizzled-related protein 4
• NCBI Official Synonym Full Names: secreted frizzled-related protein 4
• NCBI Official Symbol: SFRP4
• NCBI Official Synonym Symbols: FRP-4; FRPHE; sFRP-4
• NCBI Protein Information: secreted frizzled-related protein 4
• UniProt Protein Name: Secreted frizzled-related protein 4
• Protein Family: Secreted frizzled-related protein
• UniProt Gene Name: SFRP4
• UniProt Synonym Gene Names: FRPHE; sFRP-4; FrpHE
• UniProt Entry Name: SFRP4_HUMAN

NCBI Description

Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. The expression of SFRP4 in ventricular myocardium correlates with apoptosis related gene expression. [provided by RefSeq, Jul 2008]

Uniprot Description

SFRP4: Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP4 may act as a regulator of adult uterine morphology and function. Increases apoptosis during ovulation possibly through modulation of FZ1/FZ4/WNT4 signaling. Has phosphaturic effects by specifically inhibiting sodium-dependent phosphate uptake. Belongs to the secreted frizzled-related protein (sFRP) family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 7p14.1

Cellular Component: cell surface; cytoplasm; extracellular space; integral to membrane; nucleus

Molecular Function: G-protein coupled receptor activity; protein binding; Wnt receptor activity; Wnt-protein binding

Biological Process: cell differentiation; G-protein coupled receptor protein signaling pathway; multicellular organismal development; negative regulation of cell proliferation; negative regulation of transcription factor activity; phosphate ion homeostasis; positive regulation of apoptosis; positive regulation of epidermal cell differentiation; positive regulation of receptor internalization; response to hormone stimulus; Wnt receptor signaling pathway through beta-catenin