Western Blot (WB)
(Western blotAll lanes: TMEM53 antibody at 5ug/ml+Mouse brain tissueGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 32,24 kDaObserved band size: 32 kDa)
Rabbit anti-Human, Mouse TMEM53 Polyclonal Antibody | anti-TMEM53 antibody
TMEM53 Antibody
Gene Names
TMEM53; NET4
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
>95%, Protein G purified
Synonyms
TMEM53; Polyclonal Antibody; TMEM53 Antibody; Transmembrane protein 53; anti-TMEM53 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Sequence Length
204
Applicable Applications for anti-TMEM53 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:2000
IHC: 1:20-1:200
IF: 1:50-1:200
IHC: 1:20-1:200
IF: 1:50-1:200
Immunogen
Recombinant Human Transmembrane protein 53 protein (1-170AA)
Conjugation
Non-conjugated
Storage Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-165744
Immunogen Species
Homo sapiens (Human)
Research Area
Cell Biology
Target Names
TMEM53
Preparation and Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Western Blot (WB)
(Western blotAll lanes: TMEM53 antibody at 5ug/ml+Mouse brain tissueGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 32,24 kDaObserved band size: 32 kDa)
Western Blot (WB)
(Western blotAll lanes: TMEM53 antibody at 5ug/ml+Mouse brain tissueGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 32,24 kDaObserved band size: 32 kDa)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human pancreatic cancer using MBS7005655 at dilution of 1:100)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human pancreatic cancer using MBS7005655 at dilution of 1:100)
Immunohistochemistry (IHC)
(Immunofluorescent analysis of A549 cells using MBS7005655 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Immunohistochemistry (IHC)
(Immunofluorescent analysis of A549 cells using MBS7005655 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Related Product Information for anti-TMEM53 antibody
Nucleus
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
transmembrane protein 53 isoform 2
NCBI Official Synonym Full Names
transmembrane protein 53
NCBI Official Symbol
TMEM53
NCBI Official Synonym Symbols
NET4
NCBI Protein Information
transmembrane protein 53
UniProt Protein Name
Transmembrane protein 53
Protein Family
UniProt Gene Name
TMEM53
UniProt Entry Name
TMM53_HUMAN
Uniprot Description
TMEM53: Belongs to the TMEM53 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 1p34.1
Cellular Component: integral to membrane; nucleus
Similar Products
Product Notes
The TMEM53 tmem53 (Catalog #AAA7005655) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TMEM53 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TMEM53 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF) . WB: 1:500-1:2000 IHC: 1:20-1:200 IF: 1:50-1:200. Researchers should empirically determine the suitability of the TMEM53 tmem53 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TMEM53, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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