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Western Blot (WB) (Positive WB detected in: U87 whole cell lysate, PC-3 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysateAll lanes: PPP2R2D antibody at 3.3 μg/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 53 kDaObserved band size: 53 kDa)

Rabbit anti-Human PPP2R2D Polyclonal Antibody | anti-PPP2R2D antibody

PPP2R2D Antibody

Gene Names
PPP2R2D; MDS026
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
>95%, Protein G purified
Synonyms
PPP2R2D; Polyclonal Antibody; PPP2R2D Antibody; Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B delta isoform; PP2A subunit B isoform B55-delta; PP2A subunit B isoform PR55-delta; PP2A subunit B isoform R2-delta; PP2A subunit B i; KIAA1541; anti-PPP2R2D antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Sequence Length
288
Applicable Applications for anti-PPP2R2D antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:1000-1:5000
IHC: 1:20-1:200
IF: 1:50-1:200
Immunogen
Recombinant Human Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B delta isoform protein (1-192AA).
Immunogen Species
Homo sapiens (Human)
Research Area
Cell Biology
Target Names
PPP2R2D
Conjugate
Non-conjugated
Storage Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-18330
Preparation and Storage
Upon receipt, aliquot and store at -20°C or -80°C. Avoid repeated freeze.

Western Blot (WB)

(Positive WB detected in: U87 whole cell lysate, PC-3 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysateAll lanes: PPP2R2D antibody at 3.3 μg/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 53 kDaObserved band size: 53 kDa)

Western Blot (WB) (Positive WB detected in: U87 whole cell lysate, PC-3 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysateAll lanes: PPP2R2D antibody at 3.3 μg/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 53 kDaObserved band size: 53 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human prostate cancer using MBS7043885 at dilution of 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human prostate cancer using MBS7043885 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human glioma cancer using MBS7043885 at dilution of 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human glioma cancer using MBS7043885 at dilution of 1:100)

Immunofluorescence (IF)

(Immunofluorescent analysis of HeLa cells using MBS7043885 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Immunofluorescence (IF) (Immunofluorescent analysis of HeLa cells using MBS7043885 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Related Product Information for anti-PPP2R2D antibody
B regulatory subunit of protein phosphatase 2A (PP2A) that plays a key role in cell cycle by controlling mitosis entry and exit. The activity of PP2A complexes containing PPP2R2D (PR55-delta) fluctuate during the cell cycle: the activity is high in interphase and low in mitosis. During mitosis, activity of PP2A is inhibited via interaction with phosphorylated ENSA and ARPP19 inhibitors. Within the PP2A complexes, the B regulatory subunits modulate substrate selectivity and catalytic activity, and also may direct the localization of the catalytic enzyme to a particular subcellular compartment (By similarity).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B delta isoform isoform b
NCBI Official Synonym Full Names
protein phosphatase 2 regulatory subunit Bdelta
NCBI Official Symbol
PPP2R2D
NCBI Official Synonym Symbols
MDS026
NCBI Protein Information
serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B delta isoform
UniProt Protein Name
Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B delta isoform
UniProt Gene Name
PPP2R2D
UniProt Synonym Gene Names
KIAA1541
UniProt Entry Name
2ABD_HUMAN

Uniprot Description

PPP2R2D: B regulatory subunit of protein phosphatase 2A (PP2A) that plays a key role in cell cycle by controlling mitosis entry and exit. The activity of PP2A complexes containing PPP2R2D (PR55- delta) fluctuate during the cell cycle: the activity is high in interphase and low in mitosis. During mitosis, activity of PP2A is inhibited via interaction with phosphorylated ENSA and ARPP19 inhibitors. Within the PP2A complexes, the B regulatory subunits modulate substrate selectivity and catalytic activity, and also may direct the localization of the catalytic enzyme to a particular subcellular compartment. Belongs to the phosphatase 2A regulatory subunit B family.

Protein type: Protein phosphatase, regulatory subunit

Chromosomal Location of Human Ortholog: 10q26.3

Cellular Component: protein phosphatase type 2A complex

Molecular Function: protein phosphatase type 2A regulator activity

Biological Process: exit from mitosis; mitosis

Research Articles on PPP2R2D

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Product Notes

The PPP2R2D ppp2r2d (Catalog #AAA7043885) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PPP2R2D Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PPP2R2D can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:1000-1:5000 IHC: 1:20-1:200 IF: 1:50-1:200. Researchers should empirically determine the suitability of the PPP2R2D ppp2r2d for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPP2R2D, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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