Mouse RPN1 Monoclonal Antibody | anti-RPN1 antibody

RPN1 (ribophorin I, DKFZp686B16177, OST1, RBPH1) (HRP)

Cat. Num. AAA6182431

• Gene Names: RPN1; OST1; RBPH1
• Applications: Immunofluorescence
• Purity: Purified
• Synonyms: RPN1; Monoclonal Antibody; RPN1 (ribophorin I; DKFZp686B16177; OST1; RBPH1) (HRP); ribophorin I; RBPH1; anti-RPN1 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 4B1-1E6
• Specificity: Recognizes RPN1.
• Purity/Purification: Purified
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
• Sequence Length: 607

• Applicable Applications for anti-RPN1 antibody: Immunofluorescence (IF)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: RPN1 (AAH10839, 24aa-607aa) full-length recombinant protein with GST tag. MW of the GST tag alone is 26kD.
• Conjugate: HRP
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

(Detection limit for recombinant GST tagged RPN1 is 0.1 ng/ml as a capture antibody.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to RPN1 on HeLa cell. [antibody concentration 10 ug/ml])

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to RPN1 on HeLa cell. [antibody concentration 10 ug/ml])

Product Categories/Family for anti-RPN1 antibody

Antibodies; Enzymes; Glycosyltransferase

NCBI and Uniprot Product Information

• NCBI GI #: 14789968
• NCBI GeneID: 6184
• NCBI Official Full Name: Ribophorin I
• NCBI Official Synonym Full Names: ribophorin I
• NCBI Official Symbol: RPN1
• NCBI Official Synonym Symbols: OST1; RBPH1
• NCBI Protein Information: dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1
• Protein Family: 26S proteasome regulatory

NCBI Description

This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome. [provided by RefSeq, Jul 2008]