PAI-1 active protein

Human PAI-1

Cat. Num. AAA696178

• Gene Names: SERPINE1; PAI; PAI1; PAI-1; PLANH1
• Reactivity: Species Reactivity: Human
• Purity: Above 95% as determined by SDS-PAGE Analysis.
• Synonyms: PAI-1; Human PAI-1; Recombinant Human PAI-1; PAI-1 active protein

• Host: E Coli
• Reactivity: Species Reactivity: Human
• Purity/Purification: Above 95% as determined by SDS-PAGE Analysis.
• Sequence: VHHPPSYVA HLASDFGVR VFQQVAQAS KDRNVVFSP YGVASVLAM LQLTTGGET QQQIQAAMG FKIDDKGMA PALRHLYKE LMGPWNKDE ISTTDAIFV QRDLKLVQG FMPHFFRLF RSTVKQVDF SEVERARFI INDWVKTHT KGMISNLLG KGAVDQLTR LVLVNALYF NGQWKTPFP DSSTHRRLF HKSDGSTVS VPMMAQTNK FNYTEFTTP DGHYYDILE LPYHGDTLS MFIAAPYEK EVPLSALTN ILSAQLISH WKGNMTRLP RLLVLPKFS LETEVDLRK PLENLGMTD MFRQFQADF TSLSDQEPL HVAQALQKV KIEVNESGT VASSSTAVI VSARMAPEE IIMDRPFLF VVRHNPTGT VLFMGQVME P

• Biological Activity: Determined by its inhibitory effect against single chain tPA induced cleavage of a chromogenic substrate in Imidazole Buffer at 37 degree C. Half maximal inhibition against 1.0 mug/ml of single chain tPA was obt
• Preparation and Storage: At -20 degree C

Testing Data

Related Product Information for PAI-1 active protein

Plasminogen Activator Inhibitor-1 (PAI-1, Serpin E1) is a member of the serpin family of serine protease inhibitors, and is the primary inhibitor of urokinase and tissue plasminogen activator (tPA). PAI-1 is expressed predominantly in adipose, liver and vascular tissues, and is also produced by certain tumor cells. Elevated levels of PAI-1 are associated with obesity, diabetes and cardiovascular disease, and increased production of PAI-1 is induced by various obesity related factors such as TNF alpha , glucose, insulin, and very-low-density lipoprotein. The obesity related elevation of PAI-1 levels along with the consequential deficiency in plasminogen activators can lead directly to increased risk of thrombosis and other coronary diseases. Accordingly, PAI-1 has been implicated as an important molecular link between obesity and coronary disease. PAI-1 can also specifically bind vitronectin (VTN) to form a stable active complex with an increased circulatory half life relative to free PAI-1. Recombinant human PAI-1 is a 42.7 kDa protein containing 379 amino acid residues.

Product Categories/Family for PAI-1 active protein

Cancer; Angiogenesis; Metabolic Regulation and Diabetes

NCBI and Uniprot Product Information

• NCBI GI #: 129576
• NCBI GeneID: 5054
• UniProt Accession #: P05121; B7Z4S0; F8WD53
• Molecular Weight: 43,404 Da
• NCBI Official Full Name: Plasminogen activator inhibitor 1
• NCBI Official Synonym Full Names: serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
• NCBI Official Symbol: SERPINE1
• NCBI Official Synonym Symbols: PAI; PAI1; PAI-1; PLANH1
• NCBI Protein Information: plasminogen activator inhibitor 1; serpin E1; endothelial plasminogen activator inhibitor; serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
• UniProt Protein Name: Plasminogen activator inhibitor 1
• UniProt Gene Name: SERPINE1
• UniProt Synonym Gene Names: PAI1; PLANH1; PAI; PAI-1
• UniProt Entry Name: PAI1_HUMAN

NCBI Description

This gene encodes a member of the serine proteinase inhibitor (serpin) superfamily. This member is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]

Uniprot Description

SERPINE1: a secreted protein that acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis. Belongs to the serpin family. Interacts with VTN. Binds LRP1B; binding is followed by internalization and degradation. Plasma levels of PAI-1 and VCAM-1 together may be useful in predicting post-operative recurrence in patients with colorectal cancer.

Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: extracellular matrix; extracellular space; plasma membrane; extracellular region

Molecular Function: serine-type endopeptidase inhibitor activity; protein binding; protease binding; receptor binding

Biological Process: circadian rhythm; extracellular matrix organization and biogenesis; positive regulation of blood coagulation; negative regulation of smooth muscle cell migration; defense response to Gram-negative bacterium; positive regulation of receptor-mediated endocytosis; positive regulation of interleukin-8 production; negative regulation of fibrinolysis; platelet degranulation; transforming growth factor beta receptor signaling pathway; angiogenesis; regulation of receptor activity; chronological cell aging; negative regulation of cell migration; negative regulation of cell adhesion mediated by integrin; platelet activation; transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent; negative regulation of blood coagulation; regulation of cell proliferation; positive regulation of angiogenesis; fibrinolysis; positive regulation of transcription from RNA polymerase II promoter; gene expression; blood coagulation; positive regulation of inflammatory response

Disease: Plasminogen Activator Inhibitor-1 Deficiency