SDS-Page
( SDS-PAGE analysis of recombinant SARS-Cov2 Spike1 RBD protein fragment derived from insect cells. Sample was loaded in 15% SDS-polyacrylamide gel under reducing conditions and stained with Coomassie stain.)
COVID 19 Spike -1 RBD Coronavirus Recombinant Protein | COVID-19 recombinant protein
SARS-CoV-2 Spike-1 RBD
Purity
> 98% by SDS-PAGE & Coomassie
Synonyms
COVID 19 Spike -1 RBD Coronavirus; SARS-CoV-2 Spike-1 RBD; 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; Spike-1 RBD; COVID-19 recombinant protein
Host
Insect cells
Purity/Purification
> 98% by SDS-PAGE & Coomassie
Form/Format
lyophilized
Sequence
RVQPTDSIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFTRHHHHHH
Sequence Length
231
Tag Information
His-Tag
SDS-Page
( SDS-PAGE analysis of recombinant SARS-Cov2 Spike1 RBD protein fragment derived from insect cells. Sample was loaded in 15% SDS-polyacrylamide gel under reducing conditions and stained with Coomassie stain.)
SDS-Page
( SDS-PAGE analysis of recombinant SARS-Cov2 Spike1 RBD protein fragment derived from insect cells. Sample was loaded in 15% SDS-polyacrylamide gel under reducing conditions and stained with Coomassie stain.)
ELISA (EIA)
(Binding of sACE2-Fc to recombinant SARS-CoV2 Spike1 proteins in a functional ELISA. Recombinant SARS-CoV2 Spike1 and Spike1 RBD were coated with 1µg/ml in PBS. The sACE2-Fc [Cat# SFC-002] was added in increasing concentrations.)
ELISA (EIA)
(Binding of sACE2-Fc to recombinant SARS-CoV2 Spike1 proteins in a functional ELISA. Recombinant SARS-CoV2 Spike1 and Spike1 RBD were coated with 1µg/ml in PBS. The sACE2-Fc [Cat# SFC-002] was added in increasing concentrations.)
Related Product Information for COVID-19 recombinant protein
SARS-CoV2, which causes the global pandemic Corona virus disease 2019 (Covid-19), belongs to a family of viruses known as Corona viruses that are commonly comprised of four structural proteins: Spike protein (S), Envelope protein (E), Membrane protein (M), and Nucleocapsid protein (N). SARS-CoV2 Spike Protein (S Protein) is a glycoprotein that mediates membrane fusion and viral entry. The S protein is homotrimeric, with each ~180-kDa monomer consisting of two subunits, S1 and S2. In SARS-CoV2 proteolytic cleavage of the S protein into two distinct peptides, S1 and S2 subunits, is required for activation. The S1 subunit is focused on attachment of the protein to the host receptor while the S2 subunit is involved with cell fusion. Based on structural biology studies, the receptor binding domain (RBD), located in the C-terminal region of S1, can be oriented either in the up/standing or down/lying state. The standing state is associated with higher pathogenicity and both SARS-CoV-1 and MERS can access this state due to the flexibility in their respective RBDs. A similar two-state structure and flexibility is found in the SARS-CoV2 RBD. Based on amino acid (aa) sequence homology, the SARS-CoV2 S1 subunit RBD has 73% identity with the RBD of the SARS-CoV1 S1 RBD, but only 22% homology with the MERS S1 RBD. The low aa sequence homology is consistent with the finding that SARS and MERS bind different cellular receptors. The S Protein of the SARS-CoV2 virus, like the SARS-CoV1 counterpart, binds Angiotensin-Converting Enzyme 2 (ACE2), but with much higher affinity and faster binding kinetics.
NCBI and Uniprot Product Information
UniProt Accession #
Molecular Weight
36 kDa
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Product Notes
The COVID-19 (Catalog #AAA692572) is a Recombinant Protein produced from Insect cells and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-Tag. The amino acid sequence is listed below: RVQPTDSIVR FPNITNLCPF GEVFNATRFA SVYAWNRKRI SNCVADYSVL YNSASFSTFK CYGVSPTKLN DLCFTNVYAD SFVIRGDEVR QIAPGQTGKI ADYNYKLPDD FTGCVIAWNS NNLDSKVGGN YNYLYRLFRK SNLKPFERDI STEIYQAGST PCNGVEGFNC YFPLQSYGFQ PTNGVGYQPY RVVVLSFELL HAPATVCGPK KSTNLVKNKC VNFTRHHHHH H. It is sometimes possible for the material contained within the vial of "COVID 19 Spike -1 RBD Coronavirus, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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