Rabbit anti-Mouse, Rat C/EBP alpha Polyclonal Antibody | anti-C/EBP antibody

C/EBP alpha (Phospho-S193) Polyclonal Antibody

Cat. Num. AAA3007865

• Gene Names: Cebpa; Cebp; CBF-A; C/ebpalpha
• Reactivity: Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: C/EBP alpha; Polyclonal Antibody; C/EBP alpha (Phospho-S193) Polyclonal Antibody; CCAAT/enhancer-binding protein alpha; CEBPA; C/EBPalpha; anti-C/EBP antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: p-C/EBP alpha (S193) polyclonal antibody detects endogenous levels of C/EBP alpha protein only when phosphorylated at Ser193 (Mouse or Rat).
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 359

• Applicable Applications for anti-C/EBP antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic phosphopeptide derived from mouse C/EBP alpha around the phosphorylation site of Serine 193.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of p-C/EBP alpha (S193) polyclonal antibody at 1:500 dilutionLane2:Raw264. 7 cell lysate treated with PMA (100nM,15mins) Lane3:H9C2 ccell lysate treated with PMA (100nM,15mins))

Related Product Information for anti-C/EBP antibody

C/EBP alpha regulates gene expression in a variety of tissues including liver, adipose, lung and intestine. C/EBP alpha is a basic region/leucine zipper transcription factor selectively expressed during the differentiation of liver, adipose tissue, blood cells and the endocrine pancreas. C/EBP alpha uses a bipartite structural motif to bind DNA and appears to function exclusively in terminally differentiated, growtharrested cells. In the liver, C/EBP alpha is a transactivator of several genes, which are regulated by growth hormone. Growth hormone enhances not only the levels of C/EBP alpha mRNA and protein, but also the DNA-binding activity of C/EBP alpha. C/EBP alpha functions as an important transcription factor that regulates different genes, including Prolactin gene expression.

NCBI and Uniprot Product Information

• NCBI GI #: 86198301
• NCBI GeneID: 12606
• NCBI Accession #: NP_031704.2
• NCBI GenBank Nucleotide #: NP_031704.2
• UniProt Accession #: P53566; P05554
• Molecular Weight: ~ 37kDa
• NCBI Official Full Name: CCAAT/enhancer-binding protein alpha isoform a
• NCBI Official Synonym Full Names: CCAAT/enhancer binding protein (C/EBP), alpha
• NCBI Official Symbol: Cebpa
• NCBI Official Synonym Symbols: Cebp; CBF-A; C/ebpalpha
• NCBI Protein Information: CCAAT/enhancer-binding protein alpha
• UniProt Protein Name: CCAAT/enhancer-binding protein alpha
• UniProt Gene Name: Cebpa
• UniProt Synonym Gene Names: C/EBP alpha
• UniProt Entry Name: CEBPA_MOUSE

NCBI Description

This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain and recognizes the CCAAT motif in the promoters of target genes. The encoded protein functions in homodimers and also heterodimers with CCAAT/enhancer-binding proteins beta and gamma. Activity of this protein can modulate the expression of genes involved in cell cycle regulation as well as in body weight homeostasis. The use of alternative in-frame non-AUG (CUG) and AUG start codons results in several protein isoforms with different lengths. Differential translation initiation is mediated by an out-of-frame, upstream open reading frame which is located between the CUG and the first AUG start codons. [provided by RefSeq, Sep 2014]

Uniprot Description

C/EBP-alpha: a bZIP transcription factor which can form homodimers or heterodimers with the related proteins CEBP-beta and CEBP-gamma. Binds to the promoter and modulate the expression of the gene encoding leptin, a protein that plays an important role in body weight homeostasis. Can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells.

Protein type: DNA-binding; Transcription factor

Cellular Component: transcription factor complex; Rb-E2F complex; nuclear matrix; protein complex; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein domain specific binding; protein binding; protein homodimerization activity; DNA binding; sequence-specific DNA binding; protein heterodimerization activity; histone deacetylase binding; protein complex binding; transcription factor activity; transcription factor binding

Biological Process: transcription from RNA polymerase II promoter; fat cell differentiation; embryonic placenta development; macrophage differentiation; positive regulation of transcription, DNA-dependent; cell maturation; negative regulation of transcription from RNA polymerase II promoter; glucose homeostasis; negative regulation of cell proliferation; regulation of transcription, DNA-dependent; inner ear development; mitochondrion organization and biogenesis; cholesterol metabolic process; granulocyte differentiation; transcription, DNA-dependent; cytokine and chemokine mediated signaling pathway; liver development; regulation of cell proliferation; regulation of transcription from RNA polymerase II promoter; positive regulation of osteoblast differentiation; white fat cell differentiation; brown fat cell differentiation; positive regulation of fat cell differentiation; positive regulation of transcription from RNA polymerase III promoter; positive regulation of transcription from RNA polymerase II promoter; myeloid cell differentiation; negative regulation of transcription, DNA-dependent; lung development; urea cycle