GPR15L active protein

Human GPR15L

Cat. Num. AAA692518

• Gene Names: C10orf99; AP57; CSBF; AP-57; GPR15L; UNQ1833
• Purity: >= 98% by SDS-PAGE gel and HPLC analyses.
• Synonyms: GPR15L; Human GPR15L; Antimicrobial peptide-57; AP-57; Colon-derived SUSD2 binding factor; CSBF; UNQ1833/PRO3446; GPR15L active protein

• Host: E Coli
• Purity/Purification: >= 98% by SDS-PAGE gel and HPLC analyses.
• Form/Format: Lyophilized
• Sequence: KRRPAKAWSG RRTRLCCHRV PSPNSTNLKG HHVRLCKPCK LEPEPR LWVV PGALPQV
• Sequence Length: 81

• Species: Human
• Biological Activity: Determined by its ability to chemoattract human PBMC's in a transwell-based migration assay.
• Length (aa): 57
• Reconstitution: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
• Preparation and Storage: The lyophilized protein is stable at room tempeture for 1 month ans at 4°C for 6 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 3 months at -20°C to -80°C.

Related Product Information for GPR15L active protein

GPR15L is a newly identified ligand for GPR15, a member of the G protein-coupled receptor (GPCR) family. Upon ligation, GPR15L acts as a potent chemoattractant for GPR15-expressing T cells and together they mediate lymphocyte recruitment to the large intestine and skin. GPR15L is constitutively expressed by colon epithelial cells where its expression is minimally altered by intestinal inflammation. Conversely, GPR15L is nearly undetectable in adult epidermis but highly upregulated during wound healing and inflammation, particularly in psoriasis. Significant expression of GPR15L is also seen in additional mucosal epithelial cells, including those of the stomach, esophagus, and urinary tract. While maintaining similar expression patterns and intramolecular disulfide cysteine bridges found in members of the CC chemokine family, GPR15L differs from classic CC and CXC chemokines, whose active sites are found on the N-terminus, in that it relies on its C-terminus for receptor interaction. GPR15L was first identified as an antimicrobial peptide (AMP) due to its broad spectrum of antimicrobial activity, a property shared with many chemokines. GPR15L binds to an additional receptor, SUSD2, and early studies have indicated that both are downregulated in colon cancer tissue resulting in inhibited colon cancer growth. The E Coli-derived Recombinant Human GPR15L consists of 57 amino acid residues and has a calculated molecular weight of 6.5 kDa.

NCBI and Uniprot Product Information

• NCBI GI #: 46409376
• NCBI GeneID: 387695
• NCBI Accession #: NP_997256.1
• NCBI GenBank Nucleotide #: NM_207373.3
• UniProt Accession #: Q6UWK7.1; Q6UWK7
• Molecular Weight: 6.5 kDa
• NCBI Official Full Name: protein GPR15L
• NCBI Official Synonym Full Names: chromosome 10 open reading frame 99
• NCBI Official Symbol: C10orf99
• NCBI Official Synonym Symbols: AP57; CSBF; AP-57; GPR15L; UNQ1833
• NCBI Protein Information: protein GPR15L
• UniProt Protein Name: Protein GPR15L
• UniProt Gene Name: GPR15L
• UniProt Synonym Gene Names: AP-571 PublicationManual assertion based on opinion iniRef.4; Antimicrobial peptide-571 PublicationManual assertion based on opinion iniRef.4; CSBF1 PublicationManual assertion based on opinion iniRef.3

Uniprot Description

Chemotactic factor that mediates lymphocytes recruitement to epithelia through binding and activation of the G-protein coupled receptor GPR15 (PubMed:28900043, PubMed:28936214). May be a tumor suppressor; together with SUSD2 has a growth inhibitory effect on colon cancer cells which includes G1 cell cycle arrest (PubMed:25351403).