Irisin recombinant protein

Human Irisin

Cat. Num. AAA692299

• Gene Names: ZXDC; ZXDL
• Purity: > 90% by SDS-PAGE & HPLC analyses
• Synonyms: Irisin; Human Irisin; Fibronectin type III domain-containing protein 5 (FNDC5); Fibronectin type III repeat-containing protein 2 (FRCP2); Irisin recombinant protein

• Host: CHO cells
• Purity/Purification: > 90% by SDS-PAGE & HPLC analyses
• Form/Format: Lyophilized (10mM Sodium Phosphate, pH 7.5)
• Sequence: DSPSAPVNVT VRHLKANSAV VSWDVLEDEV VIGFAISQQK KDVRML RFIQ EVNTTTRSCA LWDLEEDTEY IVHVQAISIQ GQSPASEPVL F KTPREAEKM ASKNKDEVTM KE

• Endotoxin Level: < 0.1ng/ug of protein (1 EU/ug)
• Length (aa): 224
• Reconstitution: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1 – 1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
• Preparation and Storage: The lyophilized protein is stable at room temperature for 1 month and at 4°C for 6 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 3 months at -20°C to -80°C. Avoid repeated freeze and thaw cycles.

Related Product Information for Irisin recombinant protein

Irisin is a proteolytic hormone released into circulation by skeletal muscle tissue during acute exercise, and is a derivative of the cleaved plasma membrane protein Fibronectin type III domain-containing protein 5 (FNDC5). Found in muscle tissue, FNDC5 is synthesized at increased levels during exercise as a result of the overexpression of the exercise-responsive transcriptional co-activator PGC-1alpha (peroxisome proliferator-activated receptor-gamma co-activator-1alpha). Like its parent polypeptide, Irisin can induce the browning of subcutaneous adipocytes, or the conversion of white adipose tissue (WAT or white fat) into brown (or beige) adipose tissue (BAT or brown fat). Given that brown fat can undergo thermogenesis, or the physiologic process of heat production, Irisin contributes to the metabolic process by increasing thermogenic function and glucose homeostasis. Irisin, thus, represents a link between exercise and œburning of fats and/or sugars, and consequently may have relevance in the development of new treatments for diabetes and obesity. Recombinant Human/Murine/Rat Irisin is a glycosylated homodimer of 224 amino acid residues, whose monomer consists of 112 amino acid residues corresponding to the active portion of the 121-amino-acid length Irisin extracellular domain. Recombinant Human/Murine/Rat Irisin, appears to form a non-disulfide linked oligomeric structure in solution, has a calculated theoretical molecular weight of 25.2 kDa, but migrates at an apparent molecular weight of 28 kDa by SDS-PAGE analysis under reducing conditions due to glycosylation.

Product Categories/Family for Irisin recombinant protein

Cytokines & Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 98961138
• NCBI GeneID: 252995
• NCBI Accession #: NP_715637.2
• NCBI GenBank Nucleotide #: NM_001040653.3
• UniProt Accession #: Q8NAU1; Q6DKI8; Q7L3L1; Q8NAU2; C5J0H9; Q2QGD7
• Molecular Weight: 28 kDa
• NCBI Official Full Name: zinc finger protein ZXDC isoform 2
• NCBI Official Synonym Full Names: ZXD family zinc finger C
• NCBI Official Symbol: ZXDC
• NCBI Official Synonym Symbols: ZXDL
• NCBI Protein Information: zinc finger protein ZXDC
• UniProt Protein Name: Zinc finger protein ZXDC
• UniProt Gene Name: ZXDC
• UniProt Synonym Gene Names: ZXDL

Uniprot Description

ZXDC: Cooperates with CIITA to promote transcription of MHC class I and MHC class II genes. Belongs to the ZXD family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 3q21.3

Molecular Function: LRR domain binding; protein binding; transcription factor activity

Biological Process: positive regulation of transcription, DNA-dependent