Testing Data
(Figure: (A) SDS-PAGE (4-20%) of Recombinant Human Irisin: Recombinant Irisin Protein loaded under reducing conditions and stained with Coomassie Blue. The protein has a predicted MW of ~ 13.00kDa. (B-C) Irisin treatment stimulates UCP-1 expression in 3T3-L1 derived adipocyte. Mouse 3T3 cells were differentiated to 3T3-L1 adipocytes using the 3T3-L1 Differentiation Kit (Cat. No. MBS841812), and then incubated with active Irisin (2 ug/ml)for 5 days. Protein expressions of untreated and Irisin-treated cells were determined by (B) Western blotting (UCP-1 antibody, 1:250, Cat. No. MBS840661; Tubulin antibody, 1:500, Cat. No. MBS840661) and (C) Immunocytochemistry and lipid (Oil Red O) staining. The staining shows that active recombinant Irisin unregulated both UCP1 expression and white to brown fat conversion in mature 3T3-L1 adipocytes(active Irisin, 1~2 ug/ml; red arrow: UCP-1; orange chevron: neutral lipids within cells. Cells were counter stained with Hematoxylin using the Ready-to Use IHC/ICC kit, Cat. No. MBS841593 and Lipid (Oil Red O) Staining Kit, Cat. No. MBS841469.)
Irisin Recombinant Protein | FNDC5 recombinant protein
Irisin, Active, human recombinant
Purity
>=95%
Synonyms
Irisin; Active; human recombinant; Fibronectin Type III Domain-containing Protein 5; FNDC5 (cleaved); Fibronectin Type III Repeat-containing Protein 2; FRCP2 (cleaved).; FNDC5 recombinant protein
Host
E Coli
Purity/Purification
>=95%
Form/Format
Lyophilized from PBS. Lyophilized powder
Reconstitution Instructions
Reconstitute to 0.1 mg/ml with sterile water
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Testing Data
(Figure: (A) SDS-PAGE (4-20%) of Recombinant Human Irisin: Recombinant Irisin Protein loaded under reducing conditions and stained with Coomassie Blue. The protein has a predicted MW of ~ 13.00kDa. (B-C) Irisin treatment stimulates UCP-1 expression in 3T3-L1 derived adipocyte. Mouse 3T3 cells were differentiated to 3T3-L1 adipocytes using the 3T3-L1 Differentiation Kit (Cat. No. MBS841812), and then incubated with active Irisin (2 ug/ml)for 5 days. Protein expressions of untreated and Irisin-treated cells were determined by (B) Western blotting (UCP-1 antibody, 1:250, Cat. No. MBS840661; Tubulin antibody, 1:500, Cat. No. MBS840661) and (C) Immunocytochemistry and lipid (Oil Red O) staining. The staining shows that active recombinant Irisin unregulated both UCP1 expression and white to brown fat conversion in mature 3T3-L1 adipocytes(active Irisin, 1~2 ug/ml; red arrow: UCP-1; orange chevron: neutral lipids within cells. Cells were counter stained with Hematoxylin using the Ready-to Use IHC/ICC kit, Cat. No. MBS841593 and Lipid (Oil Red O) Staining Kit, Cat. No. MBS841469.)
Testing Data
(Figure: (A) SDS-PAGE (4-20%) of Recombinant Human Irisin: Recombinant Irisin Protein loaded under reducing conditions and stained with Coomassie Blue. The protein has a predicted MW of ~ 13.00kDa. (B-C) Irisin treatment stimulates UCP-1 expression in 3T3-L1 derived adipocyte. Mouse 3T3 cells were differentiated to 3T3-L1 adipocytes using the 3T3-L1 Differentiation Kit (Cat. No. MBS841812), and then incubated with active Irisin (2 ug/ml)for 5 days. Protein expressions of untreated and Irisin-treated cells were determined by (B) Western blotting (UCP-1 antibody, 1:250, Cat. No. MBS840661; Tubulin antibody, 1:500, Cat. No. MBS840661) and (C) Immunocytochemistry and lipid (Oil Red O) staining. The staining shows that active recombinant Irisin unregulated both UCP1 expression and white to brown fat conversion in mature 3T3-L1 adipocytes(active Irisin, 1~2 ug/ml; red arrow: UCP-1; orange chevron: neutral lipids within cells. Cells were counter stained with Hematoxylin using the Ready-to Use IHC/ICC kit, Cat. No. MBS841593 and Lipid (Oil Red O) Staining Kit, Cat. No. MBS841469.)
Related Product Information for FNDC5 recombinant protein
Irisin is a recently discovered exercise-induced myokine, which is secreted by skeletal muscles and produced by cleavage of the FNDC5. Studies show that Irisin stimulates mitochondria uncoupling protein-1 (UCP-1; a regulator of thermogenic capability of brown fat) expression, so Irisin can upregulate white to brown fat conversion and improve systemic metabolism by increasing energy expenditure. Hence, Irisin is a potential new drug target in the study of metabolic disorders, such as obesity and type 2 diabetes mellitus (T2DM). Moreover, FNDC5-like receptors are conserved in vertebrates and critical for neuronal development.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~13.00 kDa
Protein Family
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Product Notes
The FNDC5 (Catalog #AAA845464) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Irisin, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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