HGF active protein

Human HGF

Cat. Num. AAA691934

• Gene Names: HGF; SF; HGFB; HPTA; F-TCF; DFNB39
• Reactivity: Human
• Purity: > 95% by SDS-PAGE & silver stain
• Synonyms: HGF; Human HGF; Recombinant Human Hepatocyte Factor (HGF); SF; HGFB; HPTA; F-TCF; DFNB39; Hepatocyte growth factor; Scatter factor; HGF active protein

• Host: Insect Cells
• Reactivity: Human
• Purity/Purification: > 95% by SDS-PAGE & silver stain
• Form/Format: Lyophilized
• Sequence: N-Terminal Sequence: MAPARSPST
• Sequence Length: 692

• N Terminal Sequence: MAPARSPST
• Biological Activity: The activity was assayed for scattering activity in the MDCK cell assay. The ED50 was for this effect is typically at 1.0 - 5.0 ng/ml.
• Reconstitution: The lyophilized human HGF is soluble in water and acetic acid (50 mM) and can be reconstituted to a concentration of 100 ug/ml. Further dilutions should be made into buffer containing protein or medium containing serum.
• Preparation and Storage: The lyophilized HGF, though stable at room temperature, is best stored desiccated below 0 degree C. Reconstituted it should be stored in working aliquots at -20 degree C to -70 degree C. Avoid repeated freeze-thaw cycles.

Testing Data

Related Product Information for HGF active protein

Human Hepatocyte Growth Factor (HGF), also known as scatter factor, is a pleiotropic cytokine that shows homology to the enzymes of the blood coagulation cascade. It stimulates the motility and invasion of several cancer cell types and can induce angiogenesis. Recently HGF was found to be identical to scatter factor, a fibroblast-derived factor promoting the dissociation of epithelial and vascular endothelial cell colonies in monolayer cell cultures by stimulating cell migration. HGF is synthesized as a biologically inactive single chain precursor, which is cleaved by a specific, extracellular serum serine protease to a fully active heterodimer. This mature, biologically active HGF consists of a disulfide-linked alpha-beta heterodimer of the two cleavage products. Previous studies have shown that single chain and heterodimeric HGF are equally active in vitro assay systems due to either production of the serine protease in cell culture or the presence of the ubiquitous protease in serum. All biological responses induced by HGF are elicited by binding to its transmembrane tyrosine kinase receptor, which is encoded by the MET proto-oncogene. After autophosphorylation of the receptor different cytoplasmic effectors are activated that bind to the same multifunctional docking site of the receptor. HGF function is essential for normal development. Knockout studies have demonstrated that both ligand and receptor deficient mice display an embryonic lethal phenotype. Hepatocytes have to be primed before they can fully respond to HGF. This priming requires cytokines as TNF and IL-6. Recent studies have suggested that HGF synergizes with basic FGF in the induction of angiogenesis.

Product Categories/Family for HGF active protein

Cytokines & Growth Factors

References

1. Fausto N., J Hepatol 32, 19-31, (2000); 2. Lamszus K et al., Int J Dev Neurosci 17, 517-530 (1999); 3. Stella MC and Comoglio PM., Int J Biochem Cell Biol 31, 1357-1367, (1999).

NCBI and Uniprot Product Information

• NCBI GI #: 33859835
• NCBI GeneID: 3082
• NCBI Accession #: NP_000592
• NCBI GenBank Nucleotide #: NM_000601
• UniProt Accession #: P14210; Q02935; Q13494; Q14519; Q3KRB2; Q8TCE2; Q9BYL9; Q9BYM0; Q9UDU6; A1L3U6
• Molecular Weight: 78.0 kDa
• NCBI Official Full Name: hepatocyte growth factor isoform 1 preproprotein
• NCBI Official Synonym Full Names: hepatocyte growth factor (hepapoietin A; scatter factor)
• NCBI Official Symbol: HGF
• NCBI Official Synonym Symbols: SF; HGFB; HPTA; F-TCF; DFNB39
• NCBI Protein Information: hepatocyte growth factor; hepatopoeitin-A; hepatopoietin-A; lung fibroblast-derived mitogen; fibroblast-derived tumor cytotoxic factor
• UniProt Protein Name: Hepatocyte growth factor
• Protein Family: Hepatocyte growth factor
• UniProt Gene Name: HGF
• UniProt Synonym Gene Names: HPTA; SF
• UniProt Entry Name: HGF_HUMAN

NCBI Description

Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization. Ref.23 Ref.24

Subunit structure: Dimer of an alpha chain and a beta chain linked by a disulfide bond. Interacts with SRPX2; the interaction increases HGF mitogenic activity. Ref.23

Involvement in disease: Deafness, autosomal recessive, 39 (DFNB39) [MIM:608265]: A form of profound prelingual sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.19

Sequence similarities: Belongs to the peptidase S1 family. Plasminogen subfamily.Contains 4 kringle domains.Contains 1 PAN domain.Contains 1 peptidase S1 domain.

Caution: Has lost two of the three essential catalytic residues and so probably has no enzymatic activity.