Typical Testing Data/Standard Curve (for reference only)
Porcine Steroid 5 Alpha Reductase 2 ELISA Kit | SRD5a2 elisa kit
Porcine Steroid 5 Alpha Reductase 2 ELISA Kit
Reactivity
Porcine
Synonyms
Steroid 5 Alpha Reductase 2; Porcine Steroid 5 Alpha Reductase 2 ELISA Kit; SRD5a2 elisa kit
Reactivity
Porcine
Sequence Length
254
Samples
Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate
Assay Type
Quantitative Competitive
Sensitivity
1.0 ng/mL.
Preparation and Storage
Store at 2-8 degree C.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for SRD5a2 elisa kit
Intended Uses: This SRD5A1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine SRD5A1. This ELISA kit for research use only!
Principle of the Assay: SRD5A1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-SRD5A1 antibody and an SRD5A1-HRP conjugate. The assay sample and buffer are incubated together with SRD5A1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SRD5A1 concentration since SRD5A1 from samples and SRD5A1-HRP conjugate compete for the anti-SRD5A1 antibody binding site. Since the number of sites is limited, as more sites are occupied by SRD5A1 from the sample, fewer sites are left to bind SRD5A1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SRD5A1 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: SRD5A1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-SRD5A1 antibody and an SRD5A1-HRP conjugate. The assay sample and buffer are incubated together with SRD5A1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SRD5A1 concentration since SRD5A1 from samples and SRD5A1-HRP conjugate compete for the anti-SRD5A1 antibody binding site. Since the number of sites is limited, as more sites are occupied by SRD5A1 from the sample, fewer sites are left to bind SRD5A1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SRD5A1 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for SRD5a2 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
28,393 Da
NCBI Official Full Name
steroid 5-alpha-reductase 2
NCBI Official Synonym Full Names
steroid 5 alpha-reductase 2
NCBI Official Symbol
SRD5A2
NCBI Protein Information
3-oxo-5-alpha-steroid 4-dehydrogenase 2
UniProt Protein Name
3-oxo-5-alpha-steroid 4-dehydrogenase 2
Protein Family
UniProt Gene Name
SRD5A2
UniProt Synonym Gene Names
S5AR 2
NCBI Description
This gene encodes a microsomal protein expressed at high levels in androgen-sensitive tissues such as the prostate. The encoded protein is active at acidic pH and is sensitive to the 4-azasteroid inhibitor finasteride. Deficiencies in this gene can result in male pseudohermaphroditism, specifically pseudovaginal perineoscrotal hypospadias (PPSH). [provided by RefSeq, Jul 2008]
Uniprot Description
Converts testosterone (T) into 5-alpha-dihydrotestosterone (DHT) and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology.
Research Articles on SRD5a2
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Product Notes
The Porcine SRD5a2 srd5a2 (Catalog #AAA7261293) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7261293 ELISA Kit recognizes Porcine SRD5a2. It is sometimes possible for the material contained within the vial of "Steroid 5 Alpha Reductase 2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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