Dkk-3 active protein

Human Dkk-3

Cat. Num. AAA691794

• Gene Names: Dkk3; C87148; AW061014
• Reactivity: Human
• Purity: > 98% by SDS-PAGE & HPLC analyses
• Synonyms: Dkk-3; Human Dkk-3; Dickkopf-related protein-3; Dickkopf-3; REIC; Dkk-3 active protein

• Host: CHO Cells
• Reactivity: Human
• Purity/Purification: > 98% by SDS-PAGE & HPLC analyses
• Form/Format: Lyophilized
• Sequence: APAPTATSAP VKPGPALSYP QEEATLNEMF REVEELMEDT QHKLRS AVEE MEAEEAAAKA SSEVNLANLP PSYHNETNTD TKVGNNTIHV H REIHKITNN QTGQMVFSET VITSVGDEEG RRSHECIIDE DCGPSMY CQF ASFQYTCQPC RGQRMLCTRD SECCGDQLCV WGHCTKMATR GS NGTICDNQ RDCQPGLCCA FQRGLLFPVC TPLPVEGELC HDPASRLL DL ITWELEPDGA LDRCPCASGL LCQPHSHSLV YVCKPTFVGS RDQ DGEILLP REVPDEYEVG SFMEEVRQEL EDLERSLTEE MALREPAAA A AALLGGEEI
• Sequence Length: 349

• Biological Activity: Determined by its ability to inhibit alkaline phosphatase activity in differentiating MC3T3 E1 cells. The expected ED50 for this effect is 2.0 -4.0 ng/ml.

Related Product Information for Dkk-3 active protein

opment through localized inhibition of Wnt regulated processes, including anterior-posterior axial patterning, limb development, somitogenesis, and eye formation, DKKs have also been implicated post-developmentally in bone formation, bone disease, cancer, and neurodegenerative diseases. DKK proteins typically play an important regulatory role in the Wnt /beta-catenin signaling pathway by forming inhibitory complexes with LDL receptor-related proteins 5 and 6 (LRP5 and LRP6), which are essential components of the Wnt/beta-catenin signaling system. LRP5 and LRP6 are single-pass transmembrane proteins that appear to act as co-receptors for Wnt ligands involved in the Wnt/beta-catenin signaling cascade. DKK-3 has been shown to potentiate, rather than inhibit, Wnt signaling through interactions with the high-affinity, transmembrane coreceptors Kremen-1 (Krm1) and Kremen-2 (Krm2). Recombinant human DKK-3 expressed in CHO cells is a glycoprotein that has a calculated molecular weight of 36.3 kDa and contains 329 amino acid residues. Due to glycosylation, human DKK-3 migrates at an apparent molecular weight of approximately 39-49 kDa by SDS-PAGE analysis under non-reducing conditions.

Product Categories/Family for Dkk-3 active protein

Cytokines & Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 7657027
• NCBI GeneID: 50781
• NCBI Accession #: NP_056629.1
• NCBI GenBank Nucleotide #: NM_015814.2
• UniProt Accession #: Q9QUN9
• Molecular Weight: 38,388 Da
• NCBI Official Full Name: dickkopf-related protein 3
• NCBI Official Synonym Full Names: dickkopf homolog 3 (Xenopus laevis)
• NCBI Official Symbol: Dkk3
• NCBI Official Synonym Symbols: C87148; AW061014
• NCBI Protein Information: dickkopf-related protein 3; dkk-3; mDkk-3; dickkopf-3
• UniProt Protein Name: Dickkopf-related protein 3
• UniProt Gene Name: Dkk3
• UniProt Synonym Gene Names: Dickkopf-3; Dkk-3; mDkk-3
• UniProt Entry Name: DKK3_MOUSE

Uniprot Description

Function: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease

By similarity.

Subunit structure: Interacts with LRP5 and LRP6

By similarity.

Subcellular location: Secreted.

Tissue specificity: Highest expression in brain, eye and heart.

Domain: The C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6

By similarity.

Sequence similarities: Belongs to the dickkopf family.