PlGF active protein

Rat PlGF

Cat. Num. AAA691603

• Gene Names: Pgf; Plgf
• Reactivity: Rat
• Purity: > 95% by SDS-PAGE & silver stain
• Synonyms: PlGF; Rat PlGF; Recombinant Rat Placenta Growth Factor; Placenta Growth Factor; PlGF active protein

• Host: Insect Cells
• Reactivity: Rat
• Purity/Purification: > 95% by SDS-PAGE & silver stain
• Form/Format: Freeze dried
• Sequence: N-Terminal Sequence: ALSAGNNSTEMEV
• Sequence Length: 135

• N Terminal Sequence: ALSAGNNSTEMEV
• Buffer: 25 mM Tris, pH 8.5
• Preparation and Storage: The lyophilized rat PIGF, though stable at room temperature, is best stored in working aliquots at -20 degree C to -70 degree C.

Testing Data

Testing Data #2

Related Product Information for PlGF active protein

Placenta growth factor (PlGF) is a member of the PDGF/VEGF family of growth factors that share a conserved pattern of eight cysteines. Alternate splicing results in at least three human mature PlGF forms containing 131 (PlGF-1), 152 (PlGF-2), and 203 (PlGF-3) amino acids (aa) respectively. Only PlGF-2 contains a highly basic heparin-binding 21 aa insert at the C-terminus. In rat only one PlGF that is the equivalent of human PlGF-2 has been identified. Rat PlGF shares 60%, 92%, 62% and 59% aa identity with the appropriate isoform of human, mouse, canine and equine PlGF. PlGF is mainly found as variably glycosylated, secreted, 55 - 60 kDa disulfide linked homodimers. Mammalian cells expressing PlGF include villous trophoblasts, decidual cells, erythroblasts, keratinocytes and some endothelial cells. Circulating PlGF increases during human pregnancy, reaching a peak in mid-gestation; this increase is attenuated in preeclampsia. However, deletion of PlGF in the mouse does not affect development or reproduction. Postnatally, mice lacking PlGF show impaired angiogenesis in response to ischemia. PlGF binds and signals through VEGF R1/Flt-1, but not VEGF R2/Flk-1/KDR, while VEGF binds both but signals only through the angiogenic receptor, VEGF R2. PlGF and VEGF therefore compete for binding to VEGF R1, allowing high PlGF to discourage VEGF/VEGF R1 binding and promote VEGF/VEGF R2-mediated angiogenesis. However, PlGF (especially human PlGF-1) and some forms of VEGF can form dimers that decrease the angiogenic effect of VEGF on VEGF R2. PlGF-2, like VEGF164/165, shows heparin-dependent binding of neuropilin (Npn)-1 and Npn-2 and can inhibit nerve growth cone collapse. PlGF induces monocyte activation, migration, and production of inflammatory cytokines and VEGF. These activities facilitate wound and bone fracture healing, but also contribute to inflammation in active sickle cell disease and atherosclerosis. Circulating PlGF often correlates with tumor stage and aggressiveness, and therapeutic PlGF antibodies are being investigated to inhibit tumor growth and angiogenesis.

Product Categories/Family for PlGF active protein

Cytokines & Growth Factors

References

1. Osol G et al, Am J Physiol Heart Circ Physiol 294, 2008 2. Choi WS et al, J Vet Sci 6(3), 2005 3. Koh PO et al, J Vet Med Sci 69(9), 2007 4. Torry RJ et al, J Heart Lung Transplant 28(2), 2009 5. Sands M et al, Respir Res 12, 2011

NCBI and Uniprot Product Information

• NCBI GI #: 16758378
• NCBI GeneID: 94203
• NCBI Accession #: NP_446047.1
• NCBI GenBank Nucleotide #: NM_053595
• UniProt Accession #: Q63434
• Molecular Weight: 15,14 kDa
• NCBI Official Full Name: placenta growth factor
• NCBI Official Synonym Full Names: placental growth factor
• NCBI Official Symbol: Pgf
• NCBI Official Synonym Symbols: Plgf
• NCBI Protein Information: placenta growth factor
• UniProt Protein Name: Placenta growth factor
• UniProt Gene Name: Pgf
• UniProt Synonym Gene Names: Plgf; PlGF
• UniProt Entry Name: PLGF_RAT

NCBI Description

forms a heterodimer with VEGF; may play a role in vascular endothelial cell proliferation [RGD, Feb 2006]

Uniprot Description

Function: Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Also promotes cell tumor growth

By similarity.

Subunit structure: Antiparallel homodimer; disulfide-linked. Also found as heterodimer with VEGFA/VEGF. Ref.1

Subcellular location: Secreted

By similarity.

Sequence similarities: Belongs to the PDGF/VEGF growth factor family.