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ELISA (EIA) (ELISA plate was coated with purified rat IgG and IgM. Immunoglobulins were detected with Sheep Anti-Rat IgG(H+L)-BIOT followed by Streptavidin-HRP )

IgG secondary antibody

Sheep Anti-Rat IgG(H+L)-BIOT

Reactivity
Rat
Applications
ELISA
Purity
Affinity chromatography on rat IgG covalently linked to agarose
Synonyms
IgG; Polyclonal Secondary Antibody; Sheep Anti-Rat IgG(H+L)-BIOT; IgG secondary antibody
Ordering
For Research Use Only!
Host
Sheep
Reactivity
Rat
Clonality
Polyclonal
Isotype
Sheep IgG
Specificity
Reacts with the heavy and light chains of rat IgG and the light chains of rat IgM
Purity/Purification
Affinity chromatography on rat IgG covalently linked to agarose
Form/Format
Phosphate buffered saline containing <0.1% sodium azide
Concentration
0.5mg/mL (varies by lot)
Applicable Applications for IgG secondary antibody
ELISA (EIA)
Application Notes
Quality tested applications for relevant formats include-
ELISA
Source
Pooled antisera from sheep hyperimmunized with rat IgG
Conjugation
BIOT (Biotin)
Cross Adsorption
None; may react with immunoglobulins from other species and the light chains of other rat immunoglobulins
Preparation and Storage
Store at 2-8 degree C. Please refer to product specific SDS

ELISA (EIA)

(ELISA plate was coated with purified rat IgG and IgM. Immunoglobulins were detected with Sheep Anti-Rat IgG(H+L)-BIOT followed by Streptavidin-HRP )

ELISA (EIA) (ELISA plate was coated with purified rat IgG and IgM. Immunoglobulins were detected with Sheep Anti-Rat IgG(H+L)-BIOT followed by Streptavidin-HRP )

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Product Notes

The IgG (Catalog #AAA679434) is a Secondary Antibody produced from Sheep and is intended for research purposes only. The product is available for immediate purchase. The Sheep Anti-Rat IgG(H+L)-BIOT reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's IgG can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Quality tested applications for relevant formats include- ELISA. Researchers should empirically determine the suitability of the IgG for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IgG, Polyclonal Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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