Human Hepatocyte Nuclear Factor 1 Alpha ELISA Kit | HNF1a elisa kit

Human HNF1a (Hepatocyte Nuclear Factor 1 Alpha) ELISA Kit 

Cat. Num. AAA2533429

• Gene Names: HNF1A; HNF1; LFB1; TCF1; MODY3; TCF-1; HNF-1A; IDDM20
• Reactivity: Human
• Synonyms: Hepatocyte Nuclear Factor 1 Alpha; Human HNF1a (Hepatocyte Nuclear Factor 1 Alpha) ELISA Kit ; HNF1a elisa kit

• Reactivity: Human
• Specificity: This kit recognizes Human HNF1alpha in samples. No significant cross-reactivity or interference between Human HNF1alpha and analogues was observed.
• Sequence Length: 638

• Samples: Serum, plasma and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.31-20ng/mL
• Sensitivity: 0.19ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human HNF1alpha were tested 20 times on one plate, respectively.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human HNF1alpha were tested on 3 different plates, 20 replicates in each plate.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HNF1a elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human HNF1alpha concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human HNF1alpha. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HNF1alpha and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HNF1alpha, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human HNF1alpha. You can calculate the concentration of Human HNF1alpha in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 807201167
• NCBI GeneID: 6927
• NCBI Accession #: NP_001293108.1
• NCBI GenBank Nucleotide #: NM_001306179.1
• Molecular Weight: 12,491 Da
• NCBI Official Full Name: hepatocyte nuclear factor 1-alpha isoform 1
• NCBI Official Synonym Full Names: HNF1 homeobox A
• NCBI Official Symbol: HNF1A
• NCBI Official Synonym Symbols: HNF1; LFB1; TCF1; MODY3; TCF-1; HNF-1A; IDDM20
• NCBI Protein Information: hepatocyte nuclear factor 1-alpha
• UniProt Protein Name: Hepatocyte nuclear factor 1-alpha
• Protein Family: Hepatocyte nuclear factor
• UniProt Gene Name: HNF1A
• UniProt Synonym Gene Names: TCF1; HNF-1-alpha; HNF-1A; LFB1; TCF-1
• UniProt Entry Name: HNF1A_HUMAN

NCBI Description

The protein encoded by this gene is a transcription factor required for the expression of several liver-specific genes. The encoded protein functions as a homodimer and binds to the inverted palindrome 5'-GTTAATNATTAAC-3'. Defects in this gene are a cause of maturity onset diabetes of the young type 3 (MODY3) and also can result in the appearance of hepatic adenomas. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015]

Uniprot Description

HNF1A: Transcriptional activator that regulates the tissue specific expression of multiple genes, especially in pancreatic islet cells and in liver. Required for the expression of several liver specific genes. Binds to the inverted palindrome 5'- GTTAATNATTAAC-3'. Defects in HNF1A are a cause of hepatic adenomas familial (HEPAF). Hepatic adenomas are rare benign liver tumors of presumable epithelial origin that develop in an otherwise normal liver. Hepatic adenomas may be single or multiple. They consist of sheets of well-differentiated hepatocytes that contain fat and glycogen and can produce bile. Bile ducts or portal areas are absent. Kupffer cells, if present, are reduced in number and are non-functional. Conditions associated with adenomas are insulin-dependent diabetes mellitus and glycogen storage diseases (types 1 and 3). Bi-allelic inactivation of HNF1A, whether sporadic or associated with MODY3, may be an early step in the developmant of some hepatocellular carcinomas. Defects in HNF1A are the cause of maturity-onset diabetes of the young type 3 (MODY3); also symbolized MODY-3. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease. Defects in HNF1A are the cause of susceptibility to diabetes mellitus insulin-dependent type 20 (IDDM20). IDDM20 is a multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These features can result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels. Belongs to the HNF1 homeobox family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 12q24.2

Cellular Component: cytoplasm; nucleus; protein complex

Molecular Function: DNA binding; protein binding; protein dimerization activity; protein heterodimerization activity; protein homodimerization activity; transcription factor activity

Biological Process: glucose homeostasis; glucose import; insulin secretion; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter

Disease: Diabetes Mellitus, Insulin-dependent; Diabetes Mellitus, Insulin-dependent, 20; Diabetes Mellitus, Noninsulin-dependent; Hepatic Adenomas, Familial; Maturity-onset Diabetes Of The Young, Type 3; Renal Cell Carcinoma, Nonpapillary