Testing Data
(Human pancreatic carcinoma cell line MIA PaCa-2 was stainied with Mouse-Anti-Cytokeratin 18-UNLB (MBS670013); right) followed by Rat anti-mouse IgG2b-BIOT (MBS675069), streptavidin-CY3 (MBS675153))
IgG2b Secondary Antibody
Rat Anti-Mouse IgG2b-UNLB
Applications
Flow Cytometry, ELISA, Immunocytochemistry, Western Blot
Synonyms
IgG2b; Monoclonal Secondary Antibody; Rat Anti-Mouse IgG2b-UNLB; Rat Anti-Mouse IgG2b (gamma2b chain specific); IgG2b secondary antibody
Clonality
Monoclonal
Isotype
Rat (Sprague Dawley) IgG2bλ
Clone Number
SB74g
Specificity
Mouse IgG2b
Form/Format
Supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added
Applicable Applications for IgG2b secondary antibody
Flow Cytometry (FC), ELISA (EIA), FLISA, Immunocytochemistry (ICC), Western Blot (WB)
Application Notes
Flow Cytometry:
FITC and BIOT conjugates: 1.0 ug/10^6 cells
PE conjugate: =<0.1 ug/10^6 cells
For flow cytometry, the suggested use of these reagents is in a final volume of 100 uL
ELISA:
AP conjugate: 1:1,000 – 1:4,000
HRP conjugate: 1:4,000-1:8,000
BIOT conjugate: 1:5,000-1:20,000
FLISA:
FITC conjugate: 1:200-1:400
PE conjugate: <=1 ug/mL
Since applications vary, you should determine the optimum working dilution for the product that is appropriate for your specific need.
FITC and BIOT conjugates: 1.0 ug/10^6 cells
PE conjugate: =<0.1 ug/10^6 cells
For flow cytometry, the suggested use of these reagents is in a final volume of 100 uL
ELISA:
AP conjugate: 1:1,000 – 1:4,000
HRP conjugate: 1:4,000-1:8,000
BIOT conjugate: 1:5,000-1:20,000
FLISA:
FITC conjugate: 1:200-1:400
PE conjugate: <=1 ug/mL
Since applications vary, you should determine the optimum working dilution for the product that is appropriate for your specific need.
Immunogen
Mouse IgG2b hybridoma
Preparation and Storage
Store at 2-8°C.
Testing Data
(Human pancreatic carcinoma cell line MIA PaCa-2 was stainied with Mouse-Anti-Cytokeratin 18-UNLB (MBS670013); right) followed by Rat anti-mouse IgG2b-BIOT (MBS675069), streptavidin-CY3 (MBS675153))
Testing Data
(Human pancreatic carcinoma cell line MIA PaCa-2 was stainied with Mouse-Anti-Cytokeratin 18-UNLB (MBS670013); right) followed by Rat anti-mouse IgG2b-BIOT (MBS675069), streptavidin-CY3 (MBS675153))
Similar Products
Product Notes
The IgG2b (Catalog #AAA675059) is a Secondary Antibody and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's IgG2b can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC), ELISA (EIA), FLISA, Immunocytochemistry (ICC), Western Blot (WB). Flow Cytometry: FITC and BIOT conjugates: 1.0 ug/10^6 cells PE conjugate: =<0.1 ug/10^6 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 uL ELISA: AP conjugate: 1:1,000 – 1:4,000 HRP conjugate: 1:4,000-1:8,000 BIOT conjugate: 1:5,000-1:20,000 FLISA: FITC conjugate: 1:200-1:400 PE conjugate: <=1 ug/mL Since applications vary, you should determine the optimum working dilution for the product that is appropriate for your specific need. Researchers should empirically determine the suitability of the IgG2b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IgG2b, Monoclonal Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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