ARG1, aa1-322, His tag Recombinant Protein | ARG1 recombinant protein

ARG1, aa1-322, Recombinant, Human, His tag (Arginase-1, Liver-type arginase, Type I arginase)

Cat. Num. AAA636223

• Purity: Purified; ~85% (SDS-PAGE)
• Synonyms: ARG1; aa1-322; His tag; Recombinant; Human; His tag (Arginase-1; Liver-type arginase; Type I arginase); ARG1 recombinant protein

• Host: E Coli
• Purity/Purification: Purified; ~85% (SDS-PAGE)
• Form/Format: Supplied as a liquid in 20mM Tris-HCl, pH 8.0,, 2mM DTT, 100mM sodium chloride, 20% glycerol.
• Sequence: MSAKSRTIGI IGAPFSKGQP RGGVEEGPTV LRKAGLLEKL KEQECDVKDY GDLPFADIPN SPFQIVKNP RSVGKASEQL AGKVAEVKKN GRISLVLGGD HSLAIGSISG HARVHPDLGV IWVDAHTDIN PLTTTSGNL HGQPVSFLLK ELKGKIPDVP GFSWVTPCIS AKDIVYIGLR DVDPGEHYIL KTLGIKYFSM TEVDRLGIGK VMEETLSYLL GRKKRPIHLS FDVDGLDPSF TPATGTPVVG GLTYREGLYI TEEIYKTGLL SGLDIMEVNP SLGKTPEEVT RTVNTAVAIT LACFGLAREG NHKPIDYLNP PKLEHHHHHH

• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Testing Data

(15% SDS-PAG using: 3ug of A3340-13)

Related Product Information for ARG1 recombinant protein

Arginase is a manganese-containing enzyme which catalyzes the hydrolysis of arginine to ornithine and urea. It is the final enzyme of the urea cycle. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform functions in the urea cycle, and is located primarily in the cytoplasm of the liver. The type II isoform has been implicated in the regulation of the arginine/ornithine concentrations in the cell. It is located in mitochondria of several tissues in the body, with most abundance in the kidney and prostate.

Recombinant protein corresponding to aa1-322 of human ARG1 6x his tagged expressed in E.coli (NP_000036)

Product Categories/Family for ARG1 recombinant protein

Molecular Biology; MB-Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 13529083
• NCBI GeneID: 383
• UniProt Accession #: P05089; Q5JWT5; Q5JWT6; Q8TE72; Q9BS50; A6NEA0
• Molecular Weight: 35.8kD
• NCBI Official Full Name: ARG1 protein
• NCBI Official Synonym Full Names: arginase 1
• NCBI Official Symbol: ARG1
• NCBI Protein Information: arginase-1; arginase, liver; type I arginase; liver-type arginase
• UniProt Protein Name: Arginase-1
• Protein Family: Arginase
• UniProt Gene Name: ARG1
• UniProt Entry Name: ARGI1_HUMAN

NCBI Description

Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

ARG1: Homotrimer. By arginine or homoarginine. Belongs to the arginase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase; Amino Acid Metabolism - arginine and proline; EC 3.5.3.1

Chromosomal Location of Human Ortholog: 6q23

Cellular Component: extracellular space; neuron projection; cell soma; cytoplasm; nucleus; cytosol

Molecular Function: manganese ion binding; arginase activity

Biological Process: response to drug; mammary gland involution; maternal process involved in pregnancy; response to herbicide; response to amino acid stimulus; liver development; response to vitamin A; response to manganese ion; response to selenium ion; response to vitamin E; response to cadmium ion; response to zinc ion; response to methylmercury; arginine catabolic process; positive regulation of endothelial cell proliferation; response to axon injury; collagen biosynthetic process; urea cycle; response to amine stimulus; lung development

Disease: Argininemia