Mouse anti-Human Apolipoprotein H (APOH) Monoclonal Antibody | anti-APOH antibody

Monoclonal Antibody to Apolipoprotein H (APOH)

Cat. Num. AAA2111539

• Gene Names: APOH; BG; B2G1; B2GP1
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
• Purity: Protein A + Protein G Affinity Chromatography
• Synonyms: Apolipoprotein H (APOH); Monoclonal Antibody; Monoclonal Antibody to Apolipoprotein H (APOH); Apo-H; B2G1; BG; B2-GP1; B2GP1; Autoantigen beta2 GP I; Previously; Beta 2 Glycoprotein 1; APC inhibitor; Activated protein C-binding protein; Anticardiolipin cofactor; Beta-2-GPI; anti-APOH antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1 Kappa
• Clone Number: C18
• Specificity: The antibody is a mouse monoclonal antibody raised against APOH. It has been selected for its ability to recognize APOH in immunohistochemical staining and western blotting.
• Purity/Purification: Protein A + Protein G Affinity Chromatography
• Form/Format: Liquid; PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence: Thr22~Cys345

• Applicable Applications for anti-APOH antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
• Application Notes: WB: 0.5-5ug/mL; IHC: 5-30ug/mL; ICC: 5-30ug/mL
• Immunogen Sequence: Thr22~Cys345
• Cross Reactivity: Human
• Conjugation: None
• Preparation and Storage: Store at 4 degree C for frequent use. Aliquot and store at -20 degree C for 12 months.; Avoid repeated freeze/thaw cycles.; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Western Blot; Sample: Recombinant APOH, Human.)

Western Blot (WB)

(Western Blot; Sample: Lane1: Rat Heart lysate; Lane2: Rat Cerebrum lysate; Lane3: Porcine Heart lysatePrimary Ab: 1ug/ml Mouse Anti-Human APOH AntibodySecond Ab: 0.2ug/mL HRP-Linked Rabbit Anti-Mouse IgG Polyclonal Antibody )

Western Blot (WB)

(Western Blot; Sample: Lane1: Human Placenta lysate; Lane2: Human UrinePrimary Ab: 2ug/ml Mouse Anti-Human APOH AntibodySecond Ab: 0.2ug/mL HRP-Linked Rabbit Anti-Mouse IgG Polyclonal Antibody )

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Liver Tissue; Primary Ab: 30ug/ml Mouse Anti-Human APOH Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody )

Product Categories/Family for anti-APOH antibody

Metabolic Pathway; Cardiovascular Biology

NCBI and Uniprot Product Information

• NCBI GI #: 543826
• NCBI GeneID: 350
• UniProt Accession #: P02749; Q9UCN7; B2R9M3
• Molecular Weight: 345
• NCBI Official Full Name: Beta-2-glycoprotein 1
• NCBI Official Synonym Full Names: apolipoprotein H (beta-2-glycoprotein I)
• NCBI Official Symbol: APOH
• NCBI Official Synonym Symbols: BG; B2G1; B2GP1
• NCBI Protein Information: beta-2-glycoprotein 1; B2GPI; apo-H; beta(2)GPI; APC inhibitor; anticardiolipin cofactor; activated protein C-binding protein
• UniProt Protein Name: Beta-2-glycoprotein 1
• Protein Family: Beta-2-glycoprotein
• UniProt Gene Name: APOH
• UniProt Synonym Gene Names: B2G1; Apo-H; B2GPI; Beta(2)GPI
• UniProt Entry Name: APOH_HUMAN

NCBI Description

Apolipoprotein H has been implicated in a variety of physiologic pathways including lipoprotein metabolism, coagulation, and the production of antiphospholipid autoantibodies. APOH may be a required cofactor for anionic phospholipid binding by the antiphospholipid autoantibodies found in sera of many patients with lupus and primary antiphospholipid syndrome, but it does not seem to be required for the reactivity of antiphospholipid autoantibodies associated with infections. [provided by RefSeq, Jul 2008]

Uniprot Description

APOH: Binds to various kinds of negatively charged substances such as heparin, phospholipids, and dextran sulfate. May prevent activation of the intrinsic blood coagulation cascade by binding to phospholipids on the surface of damaged cells.

Protein type: Secreted; Lipid-binding; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17q24.2

Cellular Component: extracellular matrix; extracellular space; chylomicron; cell surface

Molecular Function: heparin binding; identical protein binding; protein binding; phospholipid binding; glycoprotein binding; lipid binding

Biological Process: negative regulation of angiogenesis; negative regulation of myeloid cell apoptosis; triacylglycerol metabolic process; positive regulation of blood coagulation; negative regulation of fibrinolysis; negative regulation of endothelial cell proliferation; positive regulation of lipoprotein lipase activity; negative regulation of blood coagulation; triacylglycerol transport; blood coagulation, intrinsic pathway; plasminogen activation; regulation of fibrinolysis