Mouse anti-Chicken MHC Class II Monoclonal Antibody | anti-HLA-DQB1 antibody

Mouse Anti-Chicken MHC Class II-FITC

Cat. Num. AAA673934

• Gene Names: HLA-DQB1; IDDM1; CELIAC1; HLA-DQB
• Reactivity: Chicken
• Applications: Flow Cytometry, Immunoblot, Immunohistochemistry, Immunoprecipitation, Western Blot
• Synonyms: MHC Class II; Monoclonal Antibody; Mouse Anti-Chicken MHC Class II-FITC; Mouse Anti-Chicken MHC Class II - FITC; Mouse Anti-Chicken MHC Class II; anti-HLA-DQB1 antibody

• Host: Mouse
• Reactivity: Chicken
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Clone Number: 2G11
• Specificity: Chicken MHC Class II (B-L) molecules; The chicken major histocompatibility complex (MHC), or B complex, consists of several clusters of highly polymorphic genes. Like their mammalian counterparts, the avian MHC exerts genetic influence over a variety of important biological functions such as immune response, disease resistance, growth and development, aging, and reproduction. Chicken MHC Class II genes, also known as the B-L subregion, of the chicken MHC encode cell surface glycoproteins that are homologous to mammalian Class II antigens. B-L antigens structurally are similar to mammalian Class II molecules in that they are noncovalently bound dimmers of one 32-34 kDa heavy chain and one 27-30 kDa light chain. MHC Class II is primarily expressed on B cells and antigen presenting cells (APCs).1
• Form/Format: Fluorescein (FITC) Conjugate
• Sequence Length: 229

• Applicable Applications for anti-HLA-DQB1 antibody: Flow Cytometry; Immunoblot; Immunohistochemistry; Immunoprecipitation
• Application Notes: Flow Cytometry Suggested Dilution: <= 1 ug/10^6 cells
• Characterization: To ensure optimal performance, each batch of monoclonal antibody is tested by flow cytometry to conform to characteristics of a standard reference reagent.
• Preparation and Storage: The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. ; The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. ; The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! ; Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.

References

1. Guillemot, F., P. Turmal, D. Charron, M. Le Douarin, and C. Auffray. 1986. J. Immunol. 137:1251.
2. Lawson, S., L. Rothwell, B. Lambrecht, K. Howes, K. Venugopal, and P. Kaiser. 2001. Dev. Comp. Immunol. 25:69.
3. Koritschoner, N.P., J. Madruga, S. Knespel, G. Blendinger, B. Anzinger, A. Otto, M. Zenke, and P. Bartunek. 2001. Cell Growth Differ. 12:563.
4. Katevuo, K., and O. Vainio. 1996. Immunol. 89:419.
5. Madruga, J., N. Koritschoner, S.S. Diebold, S.M. Kurz, and M. Zenke. 1999. J. Cell Sci. 112:1685.
6. Salomonsen, J., D. Dunon, K. Skjodt, D. Thorpe, O. Vainio, and J. Kaufman. 1991. Proc. Natl. Acad. Sci. USA. 88:1359.
7. Kaufman, J., K.Skjoedt, J. Salomonsen, M. Simonsen, L. Du Pasquier, R. Parisot, and P. Riegert. 1990. J. Immunol. 144:2258.

NCBI and Uniprot Product Information

• NCBI GI #: 1160938
• NCBI GeneID: 3119
• UniProt Accession #: O19707; O19506; O19632; O19709; O19710; O19711; O19712; O19714; O19715; A0AQY9
• Molecular Weight: 26,489 Da
• NCBI Official Full Name: MHC class II
• NCBI Official Synonym Full Names: major histocompatibility complex, class II, DQ beta 1
• NCBI Official Symbol: HLA-DQB1
• NCBI Official Synonym Symbols: IDDM1; CELIAC1; HLA-DQB
• NCBI Protein Information: HLA class II histocompatibility antigen, DQ beta 1 chain; MHC DQ beta; MHC class2 antigen; OTTHUMP00000029167; OTTHUMP00000178569; OTTHUMP00000178570; OTTHUMP00000178571; lymphocyte antigen; MHC class II antigen DQB1; MHC class II DQ beta chain; MHC class II antigen HLA-DQ-beta-1; MHC class II HLA-DQ beta glycoprotein; major histocompatibility complex class II beta; HLA class II histocompatibility antigen, DQ beta 1 chain-like
• UniProt Protein Name: MHC class II HLA-DQ-beta-1
• Protein Family: MHC class II transactivator
• UniProt Gene Name: HLA-DQB1
• UniProt Entry Name: O19707_HUMAN

NCBI Description

HLA-DQB1 belongs to the HLA class II beta chain paralogs. This class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and it contains six exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation. [provided by RefSeq]

Uniprot Description

Sequence similarities: Contains 1 Ig-like C1-type (immunoglobulin-like) domain. SAAS SAAS014745