Rat anti-Mouse CD104 (beta 4 Integrin) Monoclonal Antibody
Rat Anti-Mouse CD104-UNLB
Reactivity
Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Synonyms
CD104 (beta 4 Integrin); Monoclonal Antibody; Rat Anti-Mouse CD104-UNLB; Rat Anti-Mouse CD104 - Purified; Unlabeled Antibody; Rat Anti-Mouse CD104 (beta 4 Integrin); anti-CD104 (beta 4 Integrin) antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rat (Fischer 344) IgG2a kappa
Clone Number
346-11A
Specificity
Integrin beta 4 chain (CD104), Mr 200 kDa
CD104 represents the integrin beta 4 subunit that associates non-covalently with integrin alpha 6 (CD49f) to form the alpha 6 beta 4 integrin heterodimer.2, 3 The alpha 6 beta 4 complex is expressed on the basal surface of a variety of epithelial cell types, particularly on stratified squamous epithelia. It is also found in peripheral nerves, in certain subsets of endothelial cells, and on immature thymocytes, and has been found to be associated with a number of malignant tissues.3-5, 6 Hemidesmosomal alpha 6 beta 4 plays an important role in the adhesion of epithelial cells to basement membranes via interactions with laminin and/or kalinin anchoring filaments.7 It is not known if the monoclonal antibody 346-11A can block this adhesion.
CD104 represents the integrin beta 4 subunit that associates non-covalently with integrin alpha 6 (CD49f) to form the alpha 6 beta 4 integrin heterodimer.2, 3 The alpha 6 beta 4 complex is expressed on the basal surface of a variety of epithelial cell types, particularly on stratified squamous epithelia. It is also found in peripheral nerves, in certain subsets of endothelial cells, and on immature thymocytes, and has been found to be associated with a number of malignant tissues.3-5, 6 Hemidesmosomal alpha 6 beta 4 plays an important role in the adhesion of epithelial cells to basement membranes via interactions with laminin and/or kalinin anchoring filaments.7 It is not known if the monoclonal antibody 346-11A can block this adhesion.
Form/Format
Purified (UNLB) Antibody
Applicable Applications for anti-CD104 (beta 4 Integrin) antibody
Immunohistochemistry (Frozen Sections); Immunoprecipitation; Western Blot
Application Notes
Immunohistochemistry Suggested Dilution: 1-5 ug/mL
Immunogen
Tumor-associated antigen TSP-180 immunoaffinity purified from a transplantable BALB/c mouse lung cell carcinoma.^1
Characterization
To ensure lot-to-lot consistency, each batch of product is tested by immunohistochemical staining to conform to characteristics of a standard reference reagent.
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
References
1. Kennel, S.J., L.J. Foote, and K.M. Flynn. 1986. Cancer Res. 46:707.
2. Kennel, S.J., L.J. Foote, R. Falcioni, A. Sonnenberg, C.D. Stringer, C. Crouse, and M.E. Hemler. 1989. J. Biol. Chem. 264:15515.
3. Kennel, S.J., V. Godfrey, L.Y. Ch'ang, T.K. Lankford, L.J. Foote, and A Makkinje. 1992. J. Cell. Sci. 101:145.
4. Sonnenberg, A., C.J.T. Linders, J.H. Daams, and S.J. Kennel. 1990. J. Cell. Science 96:207.
5. Wadsworth, S., M.J. Halvorson, and J.E. Coligan. 1992. J. Immunol. 149:421.
6. Constantini, R.M., R. Falcioni, P. Battista, G. Zupi, S.J. Kennel, A., Colassante, I. Venturo, C.G. Curcio, and A. Sacchi. 1990. Cancer Res. 50:6107.
7. Sonnenberg, A., J. Calafat, H. Janssen, H. Daams, L.M.H. vander Raaij-Helmer, R. Falcioni, S.J. Kennel, J.D. Aplin, J. Baker, M. Loizdou, and D. Garrod. 1991. J. Cell. Biol. 113:907.
2. Kennel, S.J., L.J. Foote, R. Falcioni, A. Sonnenberg, C.D. Stringer, C. Crouse, and M.E. Hemler. 1989. J. Biol. Chem. 264:15515.
3. Kennel, S.J., V. Godfrey, L.Y. Ch'ang, T.K. Lankford, L.J. Foote, and A Makkinje. 1992. J. Cell. Sci. 101:145.
4. Sonnenberg, A., C.J.T. Linders, J.H. Daams, and S.J. Kennel. 1990. J. Cell. Science 96:207.
5. Wadsworth, S., M.J. Halvorson, and J.E. Coligan. 1992. J. Immunol. 149:421.
6. Constantini, R.M., R. Falcioni, P. Battista, G. Zupi, S.J. Kennel, A., Colassante, I. Venturo, C.G. Curcio, and A. Sacchi. 1990. Cancer Res. 50:6107.
7. Sonnenberg, A., J. Calafat, H. Janssen, H. Daams, L.M.H. vander Raaij-Helmer, R. Falcioni, S.J. Kennel, J.D. Aplin, J. Baker, M. Loizdou, and D. Garrod. 1991. J. Cell. Biol. 113:907.
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Product Notes
The CD104 (beta 4 Integrin) (Catalog #AAA673614) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Rat Anti-Mouse CD104-UNLB reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD104 (beta 4 Integrin) can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (Frozen Sections); Immunoprecipitation; Western Blot. Immunohistochemistry Suggested Dilution: 1-5 ug/mL. Researchers should empirically determine the suitability of the CD104 (beta 4 Integrin) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD104 (beta 4 Integrin), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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