Rabbit anti-Human ATXN1 Polyclonal Antibody | anti-ATXN1 antibody

ATXN1 (S776) (Ataxin-1, Spinocerebellar Ataxia Type 1 Protein, ATX1, SCA1) (APC)

Cat. Num. AAA6464475

• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: ATXN1; Polyclonal Antibody; ATXN1 (S776) (Ataxin-1; Spinocerebellar Ataxia Type 1 Protein; ATX1; SCA1) (APC); anti-ATXN1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ATXN1.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
• Sequence Length: 815

• Applicable Applications for anti-ATXN1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to amino acid residues surrounding S776 of human ATXN1 (KLH).
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-ATXN1 Antibody (S776) in CEM cell line lysates (35ug/lane). ATXN1 (arrow) was detected using the purified Pab.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of ATXN1 Antibody (S776) with hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Product Categories/Family for anti-ATXN1 antibody

Antibodies; Ataxin

References

1. Hong, S., Biochem. Biophys. Res. Commun. 371(2), 256-260 (2008). 2. Lim, J., Nature 452(7188), 713-718 (2008). 3. Krol, H.A., PLoS ONE 3(1), E1503 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 51479158
• NCBI Accession #: NP_000323
• NCBI GenBank Nucleotide #: NP_000323.2
• UniProt Accession #: P54253
• Molecular Weight: 86.923
• NCBI Official Full Name: ataxin-1 ATXN1
• UniProt Protein Name: Ataxin-1
• Protein Family: Ataxin
• UniProt Gene Name: ATXN1
• UniProt Synonym Gene Names: ATX1; SCA1
• UniProt Entry Name: ATX1_HUMAN

Uniprot Description

ataxin-1: spinocerebellar ataxia type 1 (SCA1) protein. Expansion of CAG repeats in the SCA1 gene results in an abnormally long polyglutamine tract in the protein, causing polyglutamine-induced neurodegeneration. 14-3-3 and Akt kinase stabilize both wild-type and mutant forms of ataxin-1. The expansion of the polyglutamine tract may alter this function.

Protein type: RNA-binding; Nuclear export

Chromosomal Location of Human Ortholog: 6p23

Cellular Component: nucleoplasm; nuclear RNA export factor complex; nuclear matrix; intracellular membrane-bound organelle; cytoplasm; nucleus; nuclear inclusion body

Molecular Function: protein C-terminus binding; identical protein binding; protein binding; DNA binding; protein self-association; poly(U) binding; chromatin binding; poly(rG) binding

Biological Process: RNA processing; transcription, DNA-dependent; adult locomotory behavior; nuclear export; negative regulation of insulin-like growth factor receptor signaling pathway; negative regulation of phosphorylation; visual learning; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; regulation of excitatory postsynaptic membrane potential; negative regulation of transcription, DNA-dependent; alveolus development

Disease: Spinocerebellar Ataxia 1