Testing Data
Rat anti-Mouse LPAM-1 (Integrin alpha 4 beta 7) Monoclonal Antibody
Rat Anti-Mouse LPAM-1-UNLB
Reactivity
Mouse
Applications
Flow Cytometry, Immunohistochemistry
Synonyms
LPAM-1 (Integrin alpha 4 beta 7); Monoclonal Antibody; Rat Anti-Mouse LPAM-1-UNLB; Rat Anti-Mouse LPAM-1 - Purified; Unlabeled Antibody; Rat Anti-Mouse LPAM-1 (Integrin alpha 4 beta 7); anti-LPAM-1 (Integrin alpha 4 beta 7) antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rat (Fischer) IgG2a kappa
Clone Number
DATK32
Specificity
Mouse LPAM-1 (Integrin alpha 4 beta 7)
The lymphocyte Peyer's patch adhesion molecule (LPAM-1), also known as integrin alpha 4 beta 7, is a member of the integrin family of cell surface receptors. It is expressed primarily on mucosal lymphocytes, but is also present on NK cells and eosinophils. The alpha 4 beta 7 heterodimer mediates the binding of lymphocytes to its ligand, mucosal vascular addressin (MAdCAM-1) on the high endothelial venules, thereby directing the homing of lymphocytes into Peyer's patches and intestinal lamina propria.1-4 The monoclonal antibody DATK32 recognizes a conformational epitope on the heterodimer.1 2
The lymphocyte Peyer's patch adhesion molecule (LPAM-1), also known as integrin alpha 4 beta 7, is a member of the integrin family of cell surface receptors. It is expressed primarily on mucosal lymphocytes, but is also present on NK cells and eosinophils. The alpha 4 beta 7 heterodimer mediates the binding of lymphocytes to its ligand, mucosal vascular addressin (MAdCAM-1) on the high endothelial venules, thereby directing the homing of lymphocytes into Peyer's patches and intestinal lamina propria.1-4 The monoclonal antibody DATK32 recognizes a conformational epitope on the heterodimer.1 2
Form/Format
Purified (UNLB) Antibody
Applicable Applications for anti-LPAM-1 (Integrin alpha 4 beta 7) antibody
Flow Cytometry; Immunohistochemistry (Frozen Sections)
Application Notes
Research Application(s): Blockade of binding to cell adhesion molecules
Induction of homotypic cellular aggregation
Flow Cytometry Suggested Dilution: <=1 ug/10^6 cells
Induction of homotypic cellular aggregation
Flow Cytometry Suggested Dilution: <=1 ug/10^6 cells
Immunogen
AKR/Cum mouse spontaneous T lymphoma cell line TK1
Characterization
To ensure lot-to-lot consistency, each batch of product is tested by flow cytometry to conform with characteristics of a standard reference reagent.
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
Testing Data
Testing Data
References
1. Andrew, D. P., C, Berlin, S. Honda, T. Yoshino, et al. 1994. J. Immunol. 153:3847.
2. Andrew, D. P., L.S. Rott, P.J. Kilshaw, et al. 1996. Eur J. Immunol. 26:897.
3. Hamann, A., et al. 1994. J. Immunol. 152:3282.
4. Berlin, C., E.L. Berg, M.J. Briskin, et al. 1993. Cell 74:185.
2. Andrew, D. P., L.S. Rott, P.J. Kilshaw, et al. 1996. Eur J. Immunol. 26:897.
3. Hamann, A., et al. 1994. J. Immunol. 152:3282.
4. Berlin, C., E.L. Berg, M.J. Briskin, et al. 1993. Cell 74:185.
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Product Notes
The LPAM-1 (Integrin alpha 4 beta 7) (Catalog #AAA673621) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Rat Anti-Mouse LPAM-1-UNLB reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's LPAM-1 (Integrin alpha 4 beta 7) can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry; Immunohistochemistry (Frozen Sections). Research Application(s): Blockade of binding to cell adhesion molecules Induction of homotypic cellular aggregation Flow Cytometry Suggested Dilution: <=1 ug/10^6 cells. Researchers should empirically determine the suitability of the LPAM-1 (Integrin alpha 4 beta 7) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LPAM-1 (Integrin alpha 4 beta 7), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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