Mouse anti-Human Bax Monoclonal Antibody | anti-Bax antibody
Mouse Anti-Human Bax-UNLB
Apoptosis, or programmed cell death, is a well-documented phenomenon in many cellular systems.2 It plays a key role in tissue and organ development as well as in adult tissues during cell turnover. Apoptosis can be induced by a variety of internal and external stimuli including growth factor deprivation, cytokine treatment, antigen-receptor engagement, cell-cell interactions, irradiation and glucocorticoid treatment.3 Bax is a member of the Bcl-2 family of apoptosis-associated proteins and, although it is similar in structure to Bcl-2, Bax exerts a pro-apoptotic rather than an anti-apoptotic effect on cells. Bax targets mitochondrial membranes, inducing mitochondrial damage and subsequent cell death in a caspase-independent manner, presumably via their ion channel-forming activity. These channels may then function to promote a mitochondrial permeability transition or to puncture the outer mitochondrial membrane. In addition to forming homodimer, Bax can heterodimerize with Bcl-2 and Bcl-XL proteins. This heterodimerization between pro- and anti-apoptotic family members may serve a titration function and act as a sensor for the programmed cell death program.4-13 Mab 2D2 is specific for human Bax.1
Immunohistochemistry (acetone-fixed, frozen sections and *formalin-fixed, paraffin-embedded sections)
*Heat-Induced Epitope Retrieval is required for formalin-fixed, paraffin-embedded sections
Western Blot Suggested Dilution: <=1 ug/mL
Immunohistochemistry Suggested Dilution: 5-10 ug/ml
The products should be stored at 2-8 degree C. Each product is stable for the period shown on the label if stored as directed.
2. Cohen, J.J. 1991. Adv. Immunol. 50:55.
3. Cohen, J.J., and R.C. Duke. 1992. Annu. Rev. Immunol. 10:2676.
4. Nunez, G., and M.F. Clarke. 1994. Trends Cell Biol. 4:399.
5. Cory, S. 1995. Annu. Rev. Immunol. 13:513.
6. Oltvai., Z.N., C.L. Millman, and S.J. Korsmeyer. 1993. Cell 74:609.
7. Farrow, S.N., et al. 1995. Nature 374:731.
8. Chittenden, T., et al. 1995. Nature 374:733.
9. Kiefer, M.C., et al. 1995. Nature 374:736.
10. Shimizu, S., et al. 1995. Nature 374:811.
11. Yin, X.M., Z.N. Oltvai, and S.J. Korsmeyer. 1994. Nature 369:321.
12. Cheng, E.H.-Y., et al. 1996. Nature 379:554.
13. Gottschalk, A.R., L.H. Boise, Z.N. Oltvai, M.A. Accavitti, S.J. Korsmeyer, J. Quintans, and C.B. Thompson. 1996. Cell Death and Differentiation 3:113.
NCBI and Uniprot Product Information
NCBI Description
Bcl2-related gene; involved in the regulation of apoptotic cell death [RGD]
Uniprot Description
Function: Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.
Subunit structure: Homodimer. Forms higher oligomers under stress conditions. Interacts with BCL2L11. Interaction with BCL2L11 promotes BAX oligomerization and association with mitochondrial membranes, with subsequent release of cytochrome c. Forms heterodimers with BCL2, E1B 19K protein, BCL2L1 isoform Bcl-X(L), BCL2L2, MCL1 and A1. Interacts with SH3GLB1 and HN. Interacts with SFN and YWHAZ; the interaction occurs in the cytoplasm. Under stress conditions, JNK-mediated phosphorylation of SFN and YWHAZ, releases BAX to mitochondria. Interacts with RNF144B, which regulates the ubiquitin-dependent stability of BAX. Interacts with CLU under stress conditions that cause a conformation change leading to BAX oligomerization and association with mitochondria. Does not interact with CLU in unstressed cells
Subcellular location: Isoform Alpha: Mitochondrion outer membrane; Single-pass membrane protein
By similarity. Cytoplasm
By similarity. Note: Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane
By similarity.Isoform Beta: Cytoplasm
By similarity. Isoform Gamma: Cytoplasm
Tissue specificity: Expressed in a wide variety of tissues, with highest levels in the testis and ovary.
Domain: Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family
Sequence similarities: Belongs to the Bcl-2 family.
Research Articles on Bax
Similar Products
Product Notes
The Bax bax (Catalog #AAA670007) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Human Bax-UNLB reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Bax can be used in a range of immunoassay formats including, but not limited to, ELISA; Immunohistochemistry (Acetone Fixed/Frozen Sections); Immunoprecipitation; Western Blot. Research Application(s): Immunoprecipitation Immunohistochemistry (acetone-fixed, frozen sections and *formalin-fixed, paraffin-embedded sections) *Heat-Induced Epitope Retrieval is required for formalin-fixed, paraffin-embedded sections Western Blot Suggested Dilution: <=1 ug/mL Immunohistochemistry Suggested Dilution: 5-10 ug/ml. Researchers should empirically determine the suitability of the Bax bax for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Bax, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
