Testing Data
(Fig-1: Induction of STAT4 activity by mIFN-gamma in STAT4 Leeporter Ba/F3 cells.)
STAT4 cell line
STAT4 Leeporter Luciferase Reporter-Ba/F3 Cell Line
Gene Names
STAT4; SLEB11
Applications
Functional Assay
Synonyms
STAT4; STAT4 Leeporter Luciferase Reporter-Ba/F3 Cell Line; STAT4 Reporter Ba/F3 Cell Line; Signal Transducer and Activator of Transcription; STAT4 reporter; STAT4 luciferase reporter; STAT4 cell line; Signal Transducer and Activator of Transcription-4 cell line; STAT4 reporter cell line; cell line; stable cell line; reporter cell line; luciferase; luciferase reporter cell line; leeporter; leeporter(TM); luciferase reporter; luciferase cell line; Ba/F3 cell line; Ba/F4 cell line; luciferase assay
Form/Format
Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.
Sequence Length
91
Applicable Applications for STAT4 cell line
Functional Assay
Application Notes
Monitor the STAT4 signaling pathway activity. Screen for activators or inhibitors of the STAT4 signaling pathway. Culture conditions: Cells should be grown at 37 degree C with 5% CO2 using RPMI medium supplemented with 10% heat-inactivated FBS, 1 mM sodium pyruvate, 10 mM HEPES and 1% Pen/Strep, plus 5 ng/ml mIL-3 (Note: mIL-3 is essential for Ba/F3 cell maintenance), plus 3 ug/ml of Puromycin. (Note: The parental Ba/F3-Puro cell line (Part #14135CCL) is a blank vector-transfected stable cell line which also requires 3 ug/ml of Puromycin for cell culture maintenance! Puromycin can be omitted during the reporter cell assays). It is recommended to quickly thaw the frozen cells upon receipt or from liquid nitrogen in a 37 degree C water-bath, transfer to a tube containing 10 ml of growth medium without Puromycin, spin down cells, resuspend cells in pre-warmed growth medium without Puromycin, transfer resuspended cells to T25 flask and culture in 37 degree C -CO2 incubator. Monitor the cell viability by counting cells daily for 1~3 days until cells completely recover viability as cells are doubling daily. Once cells are over 90% confluent, harvest cells by centrifugation and passage cells. At first passage, switch to growth medium containing Puromycin. Cells should be split before they reach complete confluence. To passage the cells, transfer cells to a tube, spin down cells, resuspend cells and seed appropriate aliquots of cell suspension into new culture vessels. Subcultivation ration = 1:10 to 1:20 weekly. To achieve satisfactory results, cells should not be passaged over 16 times. Functional validation: A. Response of STAT4 Leeporter - Ba/F3 cells to mIFN-gamma. 1. Harvest STAT4 Leeporter - Ba/F3 cells and seed cells into a white solid-bottom 96-well microplate in 100 ul of growth medium without IL-3 at 1 x 10^5 cells/well. 2. Right after plating cells, stimulate cells with various concentrations of mouse IFN-gamma and incubate cells at 37 degree C in a CO2 incubator for 6-16 hours. 3. Add 50 ul of luciferase assay reagent per well. 4. Incubate at room temperature for 1-5 minutes and measure luminescence using a microplate luminometer.
Shipping Note
Product available only in the USA.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Immediately upon receipt, store in liquid nitrogen.
Testing Data
(Fig-1: Induction of STAT4 activity by mIFN-gamma in STAT4 Leeporter Ba/F3 cells.)
Testing Data
(Fig-1: Induction of STAT4 activity by mIFN-gamma in STAT4 Leeporter Ba/F3 cells.)
Related Product Information for STAT4 cell line
The STAT4 Leeporter Luciferase Reporter cell line is a stably transfected Ba/F3 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the STAT4 responsive promoter, so that the cell line is designed to measure the transcriptional activity of STAT4. Signal Transducer and Activator of Transcription 4 (STAT4) is a member of the STAT transcription factor family and plays a central role in generating inflammation during protective immune responses and immune-mediated diseases. The STAT4 induction by interferon gamma is shown in Figure 1.
Product Categories/Family for STAT4 cell line
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
STAT4, partial
NCBI Official Synonym Full Names
signal transducer and activator of transcription 4
NCBI Official Symbol
STAT4
NCBI Official Synonym Symbols
SLEB11
NCBI Protein Information
signal transducer and activator of transcription 4
Protein Family
NCBI Description
The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is essential for mediating responses to IL12 in lymphocytes, and regulating the differentiation of T helper cells. Mutations in this gene may be associated with systemic lupus erythematosus and rheumatoid arthritis. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Aug 2011]
Research Articles on STAT4
Similar Products
Product Notes
The STAT4 (Catalog #AAA669345) is a Cell Line and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's STAT4 can be used in a range of immunoassay formats including, but not limited to, Functional Assay. Monitor the STAT4 signaling pathway activity. Screen for activators or inhibitors of the STAT4 signaling pathway. Culture conditions: Cells should be grown at 37 degree C with 5% CO2 using RPMI medium supplemented with 10% heat-inactivated FBS, 1 mM sodium pyruvate, 10 mM HEPES and 1% Pen/Strep, plus 5 ng/ml mIL-3 (Note: mIL-3 is essential for Ba/F3 cell maintenance), plus 3 ug/ml of Puromycin. (Note: The parental Ba/F3-Puro cell line (Part #14135CCL) is a blank vector-transfected stable cell line which also requires 3 ug/ml of Puromycin for cell culture maintenance! Puromycin can be omitted during the reporter cell assays). It is recommended to quickly thaw the frozen cells upon receipt or from liquid nitrogen in a 37 degree C water-bath, transfer to a tube containing 10 ml of growth medium without Puromycin, spin down cells, resuspend cells in pre-warmed growth medium without Puromycin, transfer resuspended cells to T25 flask and culture in 37 degree C -CO2 incubator. Monitor the cell viability by counting cells daily for 1~3 days until cells completely recover viability as cells are doubling daily. Once cells are over 90% confluent, harvest cells by centrifugation and passage cells. At first passage, switch to growth medium containing Puromycin. Cells should be split before they reach complete confluence. To passage the cells, transfer cells to a tube, spin down cells, resuspend cells and seed appropriate aliquots of cell suspension into new culture vessels. Subcultivation ration = 1:10 to 1:20 weekly. To achieve satisfactory results, cells should not be passaged over 16 times. Functional validation: A. Response of STAT4 Leeporter - Ba/F3 cells to mIFN-gamma. 1. Harvest STAT4 Leeporter - Ba/F3 cells and seed cells into a white solid-bottom 96-well microplate in 100 ul of growth medium without IL-3 at 1 x 10^5 cells/well. 2. Right after plating cells, stimulate cells with various concentrations of mouse IFN-gamma and incubate cells at 37 degree C in a CO2 incubator for 6-16 hours. 3. Add 50 ul of luciferase assay reagent per well. 4. Incubate at room temperature for 1-5 minutes and measure luminescence using a microplate luminometer. Researchers should empirically determine the suitability of the STAT4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "STAT4, Cell Line" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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