Testing Data
(Fig-1: Induction of STAT3 activity by mIFN-gamma in STAT3 Leeporter Ba/F3 cells.)
STAT3 cell line
STAT3 Leeporter Luciferase Reporter-Ba/F3 Cell Line
Gene Names
STAT3; APRF; HIES; ADMIO; ADMIO1
Applications
Functional Assay
Synonyms
STAT3; STAT3 Leeporter Luciferase Reporter-Ba/F3 Cell Line; STAT3 Reporter Ba/F3 Cell Line; Signal Transducer and Activator of Transcription; STAT3 reporter; STAT3 luciferase reporter; STAT3 cell line; Signal Transducer and Activator of Transcription-3 cell line; STAT3 reporter cell line; cell line; stable cell line; reporter cell line; luciferase; luciferase reporter cell line; leeporter; leeporter(TM); luciferase reporter; luciferase cell line; Ba/F3 cell line; stat3 cell line; luciferase assay
Form/Format
Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.
Sequence Length
51
Applicable Applications for STAT3 cell line
Functional Assay
Application Notes
Monitor the STAT3 signaling pathway activity.
Screen for activators or inhibitors of the STAT3 signaling pathway.
Culture conditions: Cells should be grown at 37 degree C with 5% CO2 using RPMI medium supplemented with 10% heat-inactivated FBS, 1 mM sodium pyruvate, 10 mM HEPES and 1% Pen/Strep, plus 5 ng/ml mIL-3 (Note: mIL-3 is essential for Ba/F3 cell maintenance), plus 3 ug/ml of Puromycin. (Note: The parental Ba/F3-Puro cell line (Part #14135CCL) is a blank vector-transfected stable cell line which also requires 3 ug/ml of Puromycin for cell culture maintenance! Puromycin can be omitted during the reporter cell assays). It is recommended to quickly thaw the frozen cells upon receipt or from liquid nitrogen in a 37 degree C water-bath, transfer to a tube containing 10 ml of growth medium without Puromycin, spin down cells, resuspend cells in pre-warmed growth medium without Puromycin, transfer resuspended cells to T25 flask and culture in 37 degree C -CO2 incubator. Monitor the cell viability by counting cells daily for 1~3 days until cells completely recover viability as cells are doubling daily. Once cells are over 90% confluent, harvest cells by centrifugation and passage cells. At first passage, switch to growth medium containing Puromycin. Cells should be split before they reach complete confluence. To passage the cells, transfer cells to a tube, spin down cells, resuspend cells and seed appropriate aliquots of cell suspension into new culture vessels. Subcultivation ration = 1:10 to 1:20 weekly. To achieve satisfactory results, cells should not be passaged over 16 times. Functional validation: A. Response of STAT3 Leeporter - Ba/F3 cells to mIFN-gamma. 1. Harvest STAT3 Leeporter - Ba/F3 cells and seed cells into a white solid-bottom 96-well microplate in 100 ul of growth medium without IL-3 at 1 x 10^5 cells/well. 2. Right after plating cells, stimulate cells with various concentrations of mouse IFN-gamma and incubate cells at 37 degree C in a CO2 incubator for 6-16 hours. 3. Add 50 ul of luciferase assay reagent per well. 4. Incubate at room temperature for 1-5 minutes and measure luminescence using a microplate luminometer
Screen for activators or inhibitors of the STAT3 signaling pathway.
Culture conditions: Cells should be grown at 37 degree C with 5% CO2 using RPMI medium supplemented with 10% heat-inactivated FBS, 1 mM sodium pyruvate, 10 mM HEPES and 1% Pen/Strep, plus 5 ng/ml mIL-3 (Note: mIL-3 is essential for Ba/F3 cell maintenance), plus 3 ug/ml of Puromycin. (Note: The parental Ba/F3-Puro cell line (Part #14135CCL) is a blank vector-transfected stable cell line which also requires 3 ug/ml of Puromycin for cell culture maintenance! Puromycin can be omitted during the reporter cell assays). It is recommended to quickly thaw the frozen cells upon receipt or from liquid nitrogen in a 37 degree C water-bath, transfer to a tube containing 10 ml of growth medium without Puromycin, spin down cells, resuspend cells in pre-warmed growth medium without Puromycin, transfer resuspended cells to T25 flask and culture in 37 degree C -CO2 incubator. Monitor the cell viability by counting cells daily for 1~3 days until cells completely recover viability as cells are doubling daily. Once cells are over 90% confluent, harvest cells by centrifugation and passage cells. At first passage, switch to growth medium containing Puromycin. Cells should be split before they reach complete confluence. To passage the cells, transfer cells to a tube, spin down cells, resuspend cells and seed appropriate aliquots of cell suspension into new culture vessels. Subcultivation ration = 1:10 to 1:20 weekly. To achieve satisfactory results, cells should not be passaged over 16 times. Functional validation: A. Response of STAT3 Leeporter - Ba/F3 cells to mIFN-gamma. 1. Harvest STAT3 Leeporter - Ba/F3 cells and seed cells into a white solid-bottom 96-well microplate in 100 ul of growth medium without IL-3 at 1 x 10^5 cells/well. 2. Right after plating cells, stimulate cells with various concentrations of mouse IFN-gamma and incubate cells at 37 degree C in a CO2 incubator for 6-16 hours. 3. Add 50 ul of luciferase assay reagent per well. 4. Incubate at room temperature for 1-5 minutes and measure luminescence using a microplate luminometer
Shipping Note
Product available only in the USA.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Functional Validation
A response of STAT3 Leeporter~Ba/F3 cells to mIFN-gamma
1: Harvest STAT3 Leeporter ~ Ba/F3 cells and seed cells into white solid-bottom 96-well microplate in 100 ul of growth medium without IL-3 at 1 x 10^5 cells/well.
2: Right after plating cells , stimulate cells with various concentrations of mouse IFN-gamma and incubate cells at 37°C in CO2 incubator for 16 hours.
3: Equilibrate the plate to room tempeture for 10 minutes.
4: Add 50ul of luciferase assay reagent (MBS668917; Refer to the reagent datasheet for detailed luciferase assay protocol) per well.
5: Read the plate in 1-5 minutes to mesure luminescence using a microplate luminometer.
1: Harvest STAT3 Leeporter ~ Ba/F3 cells and seed cells into white solid-bottom 96-well microplate in 100 ul of growth medium without IL-3 at 1 x 10^5 cells/well.
2: Right after plating cells , stimulate cells with various concentrations of mouse IFN-gamma and incubate cells at 37°C in CO2 incubator for 16 hours.
3: Equilibrate the plate to room tempeture for 10 minutes.
4: Add 50ul of luciferase assay reagent (MBS668917; Refer to the reagent datasheet for detailed luciferase assay protocol) per well.
5: Read the plate in 1-5 minutes to mesure luminescence using a microplate luminometer.
Preparation and Storage
Immediately upon receipt, store in liquid nitrogen.
Testing Data
(Fig-1: Induction of STAT3 activity by mIFN-gamma in STAT3 Leeporter Ba/F3 cells.)
Testing Data
(Fig-1: Induction of STAT3 activity by mIFN-gamma in STAT3 Leeporter Ba/F3 cells.)
Related Product Information for STAT3 cell line
The STAT3 Leeporter Luciferase Reporter cell line is a stably transfected Ba/F3 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the STAT3 responsive promoter, so that the cell line is designed to measure the transcriptional activity of STAT3. As a transcription factor, Signal Transducer and Activator of Transcription 3 (STAT3) is activated through phosphorylation at tyrosine 705 in response to various cytokines. The STAT3 induction by interferon gamma is shown in Figure 1.
Product Categories/Family for STAT3 cell line
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
STAT3, partial
NCBI Official Synonym Full Names
signal transducer and activator of transcription 3
NCBI Official Symbol
STAT3
NCBI Official Synonym Symbols
APRF; HIES; ADMIO; ADMIO1
NCBI Protein Information
signal transducer and activator of transcription 3
Protein Family
NCBI Description
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Mutations in this gene are associated with infantile-onset multisystem autoimmune disease and hyper-immunoglobulin E syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Sep 2015]
Research Articles on STAT3
Similar Products
Product Notes
The STAT3 (Catalog #AAA669347) is a Cell Line and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's STAT3 can be used in a range of immunoassay formats including, but not limited to, Functional Assay. Monitor the STAT3 signaling pathway activity. Screen for activators or inhibitors of the STAT3 signaling pathway. Culture conditions: Cells should be grown at 37 degree C with 5% CO2 using RPMI medium supplemented with 10% heat-inactivated FBS, 1 mM sodium pyruvate, 10 mM HEPES and 1% Pen/Strep, plus 5 ng/ml mIL-3 (Note: mIL-3 is essential for Ba/F3 cell maintenance), plus 3 ug/ml of Puromycin. (Note: The parental Ba/F3-Puro cell line (Part #14135CCL) is a blank vector-transfected stable cell line which also requires 3 ug/ml of Puromycin for cell culture maintenance! Puromycin can be omitted during the reporter cell assays). It is recommended to quickly thaw the frozen cells upon receipt or from liquid nitrogen in a 37 degree C water-bath, transfer to a tube containing 10 ml of growth medium without Puromycin, spin down cells, resuspend cells in pre-warmed growth medium without Puromycin, transfer resuspended cells to T25 flask and culture in 37 degree C -CO2 incubator. Monitor the cell viability by counting cells daily for 1~3 days until cells completely recover viability as cells are doubling daily. Once cells are over 90% confluent, harvest cells by centrifugation and passage cells. At first passage, switch to growth medium containing Puromycin. Cells should be split before they reach complete confluence. To passage the cells, transfer cells to a tube, spin down cells, resuspend cells and seed appropriate aliquots of cell suspension into new culture vessels. Subcultivation ration = 1:10 to 1:20 weekly. To achieve satisfactory results, cells should not be passaged over 16 times. Functional validation: A. Response of STAT3 Leeporter - Ba/F3 cells to mIFN-gamma. 1. Harvest STAT3 Leeporter - Ba/F3 cells and seed cells into a white solid-bottom 96-well microplate in 100 ul of growth medium without IL-3 at 1 x 10^5 cells/well. 2. Right after plating cells, stimulate cells with various concentrations of mouse IFN-gamma and incubate cells at 37 degree C in a CO2 incubator for 6-16 hours. 3. Add 50 ul of luciferase assay reagent per well. 4. Incubate at room temperature for 1-5 minutes and measure luminescence using a microplate luminometer. Researchers should empirically determine the suitability of the STAT3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "STAT3, Cell Line" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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