Mouse anti-Human C19orf2 Polyclonal Antibody | anti-URI1 antibody

C19orf2 (FLJ10575, NNX3, Protein NNX3, RMP, RNA Polymerase II Subunit 5-mediating Protein, RPB5-mediating Protein, Unconventional Prefoldin RPB5 Interactor, URI)

Cat. Num. AAA6006170

• Gene Names: URI1; RMP; URI; NNX3; C19orf2; PPP1R19
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: C19orf2; Polyclonal Antibody; C19orf2 (FLJ10575; NNX3; Protein NNX3; RMP; RNA Polymerase II Subunit 5-mediating Protein; RPB5-mediating Protein; Unconventional Prefoldin RPB5 Interactor; URI); Anti -C19orf2 (FLJ10575; anti-URI1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human C19orf2.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.
• Sequence: MWRGCARSRKRKKVDNDYNALRERLSTLPDKLSYNIMVPFGPFAFMPGKLVHTNEVTVLLGDNWFAKCSAKQAVGLVEHRKEHVRKTIDDLKKVMKNFESRVEFTEDLQKMSDAAGDIVDIREEIKCDFEFKAKHRIAHKPHSKPKTSDIFEADIANDVKSKDLLADKELWARLEELERQEELLGELDSKPDTVIANGEDTTSSEEEKEDRNTNVNAMHQVTDSHTPCHKDVASSEPFSGQVNSQLNCSVNGSSSYHSDDDDDDDDDDDDDNIDDDDGDNDHEALGVGDNSIPTIYFSHTVEPKRVRINTGKNTTLKFSEKKEEAKRKRKNSTGSGHSAQELPTIRTPADIYRAFVDVVNGEYVPRKSILKSRSRENSVCSDTSESSAAEFDDRRGVLRSISCEEATCSDTSESILEEEPQENQKKLLPLSVTPEAFSGTVIEKEFVSPSLTPPPAIAHPALPTIPERKEVLLEASEETGKRVSKFKAARLQQKD

• Applicable Applications for anti-URI1 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.
• Immunogen: Full length human C19orf2, aa1-495 (NP_604431.1).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of C19orf2 expression in transfected 293T cell line by C19orf2 polyclonal antibody. Lane 1: C19orf2 transfected lysate (54.45kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-URI1 antibody

The protein encoded by this gene binds to RNA polymerase II subunit 5 (RPB5) and negatively modulates transcription through its binding to RPB5. The encoded protein seems to have inhibitory effects on various types of activated transcription, but it requires the RPB5-binding region. This protein acts as a corepressor. It is suggested that it may require signaling processes for its function or that it negatively modulates genes in the chromatin structure. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Product Categories/Family for anti-URI1 antibody

Antibodies; Abs to KIAA; orf

NCBI and Uniprot Product Information

• NCBI GI #: 34782999
• NCBI GeneID: 8725
• UniProt Accession #: O94763; Q8TC23; Q9UNU3; A8K805; H7BY42
• Molecular Weight: 59,832 Da
• NCBI Official Full Name: C19orf2 protein, partial
• NCBI Official Synonym Full Names: URI1, prefoldin-like chaperone
• NCBI Official Symbol: URI1
• NCBI Official Synonym Symbols: RMP; URI; NNX3; C19orf2; PPP1R19
• NCBI Protein Information: unconventional prefoldin RPB5 interactor 1; RPB5-mediating protein; protein phosphatase 1 regulatory subunit 19; protein phosphatase 1, regulatory subunit 19; RNA polymerase II subunit 5-mediating protein
• UniProt Protein Name: Unconventional prefoldin RPB5 interactor 1
• Protein Family: Unconventional prefoldin RPB5 interactor
• UniProt Gene Name: URI1
• UniProt Synonym Gene Names: C19orf2; NNX3; PPP1R19; RMP; URI; RPB5-mediating protein
• UniProt Entry Name: RMP_HUMAN

NCBI Description

This gene encodes member of the prefoldin family of molecular chaperones. The encoded protein functions as a scaffolding protein and plays roles in ubiquitination and transcription, in part though interactions with the RNA polymerase II subunit RPB5. This gene may play a role in multiple malignancies including ovarian cancer and hepatocellular carcinoma. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 22. [provided by RefSeq, Nov 2011]

Uniprot Description

RMP: Involved in gene transcription regulation. Acts as a transcriptional repressor in concert with the corepressor UXT to regulate androgen receptor (AR) transcription. May act as a tumor suppressor to repress AR-mediated gene transcription and to inhibit anchorage-independent growth in prostate cancer cells. Required for cell survival in ovarian cancer cells. Together with UXT, associates with chromatin to the NKX3-1 promoter region. Antagonizes transcriptional modulation via hepatitis B virus X protein. Homodimer. Component of the URI complex that contains PFDN2, POLR2E/RPB5, RUVBL2, RUVBL1 and URI1. Interacts with PPP1CC; the interaction is phosphorylation-dependent and occurs in a growth factor-dependent manner. Interacts with PFDN2, PFDN4 and STAP1; the interactions are phosphorylation-dependent and occurs in a growth-dependent manner in the mitochondrion. Interacts (via the middle C-terminus region) with GTF2F1 and GTF2F2. Interacts with DMAP1, POLR2E/RPB5 and UXT. Ubiquitous. Expressed in ovarian cancers. Expressed strongly in skeletal muscle. Expressed weakly in brain, heart, pancreas and in prostate epithelial cells. Belongs to the RNA polymerase II subunit 5-mediating protein family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 19q12

Cellular Component: mitochondrion; dendrite; cytoplasm; DNA-directed RNA polymerase II, core complex; nucleus

Molecular Function: phosphatase inhibitor activity; protein binding; chromatin binding; protein phosphatase inhibitor activity

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; response to virus; negative regulation of transcription from RNA polymerase II promoter; regulation of cell growth