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Flow Cytometry (FC/FACS) (Fig: 1 Immunohistochemical analysis of kappa in human tonsil using kappa antibody (Clone: L1C1) at 1: 400 dilution.)

Mouse anti-Human Kappa Monoclonal Antibody | anti-Kappa antibody

Monoclonal Antibody to Kappa (Clone: L1C1)

Reactivity
Human
Applications
Immunohistochemistry
Purity
Purified
Protein G Chromatography
Synonyms
Kappa; Monoclonal Antibody; Monoclonal Antibody to Kappa (Clone: L1C1); IGKV1D-16; Ig kappa chain V-I region HK101; anti-Kappa antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1 Kappa
Clone Number
L1C1
Purity/Purification
Purified
Protein G Chromatography
Form/Format
100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Sequence Length
117
Applicable Applications for anti-Kappa antibody
Immunohistochemistry (IHC)
Application Notes
IHC analysis: 1: 400-1: 800
Antigen
Kappa
Immunogen Information
B lymphoma cells are used as immunogen for this antibody.
Conjugation
Unconjugated
Preparation and Storage
Store the antibody at 4 degree C; stable for 6 months.
For long-term storage; store at -20 degree C.
Avoid repeated freeze and thaw cycles.

Flow Cytometry (FC/FACS)

(Fig: 1 Immunohistochemical analysis of kappa in human tonsil using kappa antibody (Clone: L1C1) at 1: 400 dilution.)

Flow Cytometry (FC/FACS) (Fig: 1 Immunohistochemical analysis of kappa in human tonsil using kappa antibody (Clone: L1C1) at 1: 400 dilution.)

Immunohistochemistry (IHC)

(Fig: 2 Immunohistochemical analysis of kappa in human Placenta tissue using kappa antibody (Clone: L1C1) at 10 ug/ml.)

Immunohistochemistry (IHC) (Fig: 2 Immunohistochemical analysis of kappa in human Placenta tissue using kappa antibody (Clone: L1C1) at 10 ug/ml.)
Related Product Information for anti-Kappa antibody
The kappa light chain antibody recognizes the kappa light chain of immunoglobulin. The kappa light chain itself is one of the two small polypeptide subunits of an antibody, the other being lambda. Antibodies are produced by the B-cells in lymphoid tissue. Each B lymphocyte expresses either lambda or kappa light chain but never both together. Hence, the kappa light chain antibody is a useful marker for identifying B lymphocytes expressing kappa light chain.
Product Categories/Family for anti-Kappa antibody

NCBI and Uniprot Product Information

NCBI GI #
UniProt Accession #
Molecular Weight
12,730 Da
NCBI Official Full Name
Immunoglobulin kappa variable 1D-16
UniProt Protein Name
Immunoglobulin kappa variable 1D-16
Protein Family
UniProt Gene Name
IGKV1D-16

Uniprot Description

V region of the variable domain of immunoglobulin light chains that participates in the antigen recognition (PubMed:24600447). Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:20176268, PubMed:22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:20176268, PubMed:17576170).

Similar Products

Product Notes

The Kappa igkv1d-16 (Catalog #AAA669135) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to Kappa (Clone: L1C1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Kappa can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC). IHC analysis: 1: 400-1: 800. Researchers should empirically determine the suitability of the Kappa igkv1d-16 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Kappa, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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