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Testing Data (Figure-1: Cell surface staining of PBMC, Lymhocytes gated. 0.5 ul of Kappa antibody was used.)

Mouse anti-Human Kappa Monoclonal Antibody | anti-Kappa antibody

Monoclonal Antibody to Kappa (Clone: ABM3A47)

Reactivity
Human
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay, Western Blot
Purity
Protein G Chromatography
Synonyms
Kappa; Monoclonal Antibody; Monoclonal Antibody to Kappa (Clone: ABM3A47); Anti-Kappa Antibody; ABM3A47; IGKV1D-16; Antibody to Kappa; Kappa Antibody; Ig kappa chain V-I region HK101; anti-Kappa antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
ABM3A47
Purity/Purification
Protein G Chromatography
Form/Format
100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Sequence Length
117
Applicable Applications for anti-Kappa antibody
Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Western Blot (WB)
Application Notes
IHC: 1:400-1:800
Antigen
Kappa
Immunogen Information
B lymphoma cells are used as immunogen for this antibody.
Preparation and Storage
Store the antibody at 4 degree C, stable for 6 months.
For long-term storage, store at -20 degree C.
Avoid repeated freeze and thaw cycles.

Testing Data

(Figure-1: Cell surface staining of PBMC, Lymhocytes gated. 0.5 ul of Kappa antibody was used.)

Testing Data (Figure-1: Cell surface staining of PBMC, Lymhocytes gated. 0.5 ul of Kappa antibody was used.)

Western Blot (WB)

(Figure-2: Figure-2: Western Blot Analysis of Kappa. Anti-Kappa antibody (Clone: ABM3A47) was tested at 0.01 ug/ml on (1) h Spleen and (2) h Kidney lysates.)

Western Blot (WB) (Figure-2: Figure-2: Western Blot Analysis of Kappa. Anti-Kappa antibody (Clone: ABM3A47) was tested at 0.01 ug/ml on (1) h Spleen and (2) h Kidney lysates.)
Related Product Information for anti-Kappa antibody
The kappa light chain antibody recognizes the kappa light chain of immunoglobulin. The kappa light chain itself is one of the two small polypeptide subunits of an antibody, the other being lambda. Antibodies are produced by the B-cells in lymphoid tissue. Each B lymphocyte expresses either lambda or kappa light chain but never both together. Hence, the kappa light chain antibody is a useful marker for identifying B lymphocytes expressing kappa light chain.
Product Categories/Family for anti-Kappa antibody

NCBI and Uniprot Product Information

NCBI GI #
UniProt Accession #
NCBI Official Full Name
Immunoglobulin kappa variable 1D-16
UniProt Protein Name
Ig kappa chain V-ID region 16
Protein Family
UniProt Gene Name
IGKV1D-16
UniProt Entry Name
KV109_HUMAN

Uniprot Description

KV109:

Protein type: Immunoglobulin superfamily

Chromosomal Location of Human Ortholog: 2p12

Cellular Component: external side of plasma membrane; extracellular region; extracellular space; plasma membrane

Molecular Function: antigen binding; serine-type endopeptidase activity

Biological Process: B cell receptor signaling pathway; complement activation; complement activation, classical pathway; defense response to bacterium; innate immune response; phagocytosis, engulfment; phagocytosis, recognition; positive regulation of B cell activation; receptor-mediated endocytosis; regulation of immune response; retinal homeostasis

Disease: Immunoglobulin Kappa Light Chain Deficiency

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Product Notes

The Kappa igkv1d-16 (Catalog #AAA669308) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to Kappa (Clone: ABM3A47) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Kappa can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Western Blot (WB). IHC: 1:400-1:800. Researchers should empirically determine the suitability of the Kappa igkv1d-16 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Kappa, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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