Western Blot (WB)
(Fig: 1 Western blot analysis of Bcl-X using at ~30 kDa.)
Rabbit Bcl-xL Polyclonal Antibody | anti-Bcl-xL antibody
Polyclonal Antibody to Bcl-xL
IHC (paraffin): 1: 1000-1: 5000
IP: 1: 50-1: 200
For long-term storage, store at -20 degree C.
Avoid repeated freeze and thaw cycles.
Western Blot (WB)
(Fig: 1 Western blot analysis of Bcl-X using at ~30 kDa.)
Western Blot (WB)
(Fig: 1 Western blot analysis of Bcl-X using at ~30 kDa.)
Immunohistochemistry (IHC)
(Fig: 2 Formalin-fixed, paraffin-embedded human colon carcinoma tissue section stained for Bcl-X expression using at 1: 2000. Hematoxylin-eosin counterstain.)
Immunohistochemistry (IHC)
(Fig: 2 Formalin-fixed, paraffin-embedded human colon carcinoma tissue section stained for Bcl-X expression using at 1: 2000. Hematoxylin-eosin counterstain.)
Immunohistochemistry (IHC)
(Fig: 3 Formalin-fixed, paraffin-embedded normal human colon epithelium tissue section stained for Bcl-X expression using at 1: 2000. Hematoxylin-eosin counterstain. A course granular Bcl-X staining pattern associated with the mitochondria is observed.)
Immunohistochemistry (IHC)
(Fig: 3 Formalin-fixed, paraffin-embedded normal human colon epithelium tissue section stained for Bcl-X expression using at 1: 2000. Hematoxylin-eosin counterstain. A course granular Bcl-X staining pattern associated with the mitochondria is observed.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform. [provided by RefSeq, Jul 2008]
Uniprot Description
BAD: a proapoptotic member of the Bcl-2 family. Displaces Bax from binding to Bcl-2 and Bcl-xL, resulting in cell death. Survival factors such as IL-3 can inhibit the apoptotic activity of Bad inducing the phosphorylation of Bad by Akt and p90RSK. 14-3-3 proteins bind phosphorylated Bad, inhibiting its binding to Bcl-2 and Bcl-xL. Phosphorylation by mitochondria-anchored PKA in the BH3 domain can block the dimerization of Bad and Bcl-xL.
Protein type: Apoptosis
Chromosomal Location of Human Ortholog: 11q13.1
Cellular Component: cytosol; mitochondrial outer membrane; mitochondrion
Molecular Function: caspase activator activity; lipid binding; phospholipid binding; protein binding; protein kinase binding
Biological Process: ADP metabolic process; apoptosis; ATP metabolic process; caspase activation; glucose homeostasis; negative regulation of cytolysis; pore complex biogenesis; positive regulation of apoptosis; positive regulation of autophagy; positive regulation of caspase activity; positive regulation of epithelial cell proliferation; positive regulation of glucokinase activity; positive regulation of insulin secretion; positive regulation of proteolysis; protein insertion into mitochondrial membrane during induction of apoptosis; regulation of mitochondrial membrane permeability
Research Articles on Bcl-xL
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Product Notes
The Bcl-xL bad (Catalog #AAA668541) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Bcl-xL reacts with Dog, Gerbil, Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Bcl-xL can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Formalin/Paraffin, Immunoprecipitation (IP). WB: 1: 1000-1: 2000 IHC (paraffin): 1: 1000-1: 5000 IP: 1: 50-1: 200. Researchers should empirically determine the suitability of the Bcl-xL bad for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Bcl-xL, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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