Western Blot (WB)
(Western Blot analysis of extracts from PDGF, wortmannin and LY294002 treated NIH/3T3 cells, using A1124-02 (A); A1124-92 (B) or A1124-03 (C). D is an analysis of Akt activity in PDGF,wortmannin and LY294002 treated NIH/3T3 cells, using Akt Kinase (Rac PKa, PKBa) Assay Kit BioAssay™ (A1125-45).)
LY294002, Inhibitor
LY294002 (PI 3 Kinase Inhibitor, 2-(4-Morpholinyl)-8-phenyl-4H-1- benzopyran-4-one)
Synonyms
LY294002; LY294002 (PI 3 Kinase Inhibitor; 2-(4-Morpholinyl)-8-phenyl-4H-1- benzopyran-4-one); inhibitor
Form/Format
Supplied as a liquid in DMSO.
Application Notes
Sutiable for use in vitro and in vivo.
Dilution: Cultured cells: Recommend treating the cells with LY294002 for one hour prior to, and for the duration of, the stimulation.
Optimal dilutions to be determined by researher.
Dilution: Cultured cells: Recommend treating the cells with LY294002 for one hour prior to, and for the duration of, the stimulation.
Optimal dilutions to be determined by researher.
Molecular Formula
C19H17NO3
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Light sensitive. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western Blot analysis of extracts from PDGF, wortmannin and LY294002 treated NIH/3T3 cells, using A1124-02 (A); A1124-92 (B) or A1124-03 (C). D is an analysis of Akt activity in PDGF,wortmannin and LY294002 treated NIH/3T3 cells, using Akt Kinase (Rac PKa, PKBa) Assay Kit BioAssay™ (A1125-45).)
Western Blot (WB)
(Western Blot analysis of extracts from PDGF, wortmannin and LY294002 treated NIH/3T3 cells, using A1124-02 (A); A1124-92 (B) or A1124-03 (C). D is an analysis of Akt activity in PDGF,wortmannin and LY294002 treated NIH/3T3 cells, using Akt Kinase (Rac PKa, PKBa) Assay Kit BioAssay™ (A1125-45).)
Structure
(Structure )
Structure
(Structure )
Related Product Information for LY294002, inhibitor
LY294002 is a potent and specific cell-permeable inhibitor of phosphatidylinositol 3-kinase (PI3-kinase). LY294002 at a concentration of 50uM completely abolishes the PI3-K activity of intact neutrophils stimulated with fMet-Leu-Phe, without apparent cell toxicity. While concentration of 50uM is observed to be efficacious for inhibiting PI3-K activity in stimulated neutrophils, this concentration produces no significant inhibition of other kinases including PKC, PKA, MAP kinase, S6 kinase, EGF receptor tyrosine kinase, Src, PI4-kinase, diacylglycerol kinase, or rabbit kidney ATPase. This compound is a useful tool for identifying cellular event that are regulated by the PI3-kinase/Akt axis, and is observed to induce apoptosis in many cell types by blocking the PI3-kinase/Akt anti-apoptotic pathway.
LY294002 (PI3 Kinase Inhibitor) was shown to act in vivo as a highly selective inhibitor of phosphatidylinositol 3 (PI3) kinase. When used at a concentration of 50uM, it specifically abolished PI3 kinase activity (IC50=0.43ug/ml; 1.40uM) but did not inhibit other lipid and protein kinases such as PI4 kinase, PKC, MAP kinase or c-Src (1). LY294002 has been shown to block PI3 kinase-dependent Akt phosphorylation and kinase activity.
LY294002 (PI3 Kinase Inhibitor) was shown to act in vivo as a highly selective inhibitor of phosphatidylinositol 3 (PI3) kinase. When used at a concentration of 50uM, it specifically abolished PI3 kinase activity (IC50=0.43ug/ml; 1.40uM) but did not inhibit other lipid and protein kinases such as PI4 kinase, PKC, MAP kinase or c-Src (1). LY294002 has been shown to block PI3 kinase-dependent Akt phosphorylation and kinase activity.
Product Categories/Family for LY294002, inhibitor
Similar Products
Product Notes
The LY294002 (Catalog #AAA655326) is an Inhibitor and is intended for research purposes only. The product is available for immediate purchase. Sutiable for use in vitro and in vivo. Dilution: Cultured cells: Recommend treating the cells with LY294002 for one hour prior to, and for the duration of, the stimulation. Optimal dilutions to be determined by researher. Researchers should empirically determine the suitability of the LY294002 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LY294002, Inhibitor" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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