Western Blot (WB)
(Western blot analysis of extracts from Jurkat cells, Calyculin A or LY294002-treated, using A1124-04 Akt Phosphorylated (Ser473) Antibody A1124-04 (upper), Phospho-Akt (Thr308) Antibody A1124-04 (middle) or Akt Antibody A1124-04 (lower).)
Akt Control Cell Extracts Immunoglobulin
Akt Control Cell Extracts (Rac PKa, PKBa)
Purity
Lysate
Synonyms
Akt Control Cell Extracts; Akt Control Cell Extracts (Rac PKa; PKBa); Akt Control Cell Extracts immunoglobulin
Purity/Purification
Lysate
Form/Format
Supplied as a liquid in SDS Sample Buffer: 62.5mM Tris-HCl, pH 6.8 at 25 degree C, 2% w/v SDS, 10% glycerol, 50mM DTT, 0.01% w/v bromophenol blue or phenol red.
Preparation and Storage
-20 degree C
Western Blot (WB)
(Western blot analysis of extracts from Jurkat cells, Calyculin A or LY294002-treated, using A1124-04 Akt Phosphorylated (Ser473) Antibody A1124-04 (upper), Phospho-Akt (Thr308) Antibody A1124-04 (middle) or Akt Antibody A1124-04 (lower).)
Western Blot (WB)
(Western blot analysis of extracts from Jurkat cells, Calyculin A or LY294002-treated, using A1124-04 Akt Phosphorylated (Ser473) Antibody A1124-04 (upper), Phospho-Akt (Thr308) Antibody A1124-04 (middle) or Akt Antibody A1124-04 (lower).)
Related Product Information for Akt Control Cell Extracts immunoglobulin
Akt, also referred to as PKB or Rac, plays a critical role in controlling the balance between survival and apoptosis (1-3). This protein kinase is activated by insulin and various growth and survival factors and functions in a wortmannin-sensitive pathway involving PI3 kinase (2,3). Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (4) and also by phosphorylation within the C-terminus at Ser473. Akt functions to promote cell survival by inhibiting apoptosis by means of its ability to phosphorylate and inactivate several targets, including Bad (5), Forkhead transcription factors (6) and caspase-9. PTEN phosphatase is a major negative regulator of the PI3 kinase/Akt signaling pathway (7). LY294002 is a specific PI3 kinase inhibitor (8). Nonphosphorylated Akt Cell Extracts: Total cell extracts from Jurkat cells, serum starved for 2 hours with 50uM LY294002 treatment during the second hour, serve as a negative control.
Product Categories/Family for Akt Control Cell Extracts immunoglobulin
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Product Notes
The Akt Control Cell Extracts (Catalog #AAA652623) is an Immunoglobulin and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Akt Control Cell Extracts, Immunoglobulin" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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